PMID: 9176079Apr 1, 1997Paper

Effect of shigella enterotoxin 1 (ShET1) on rabbit intestine in vitro and in vivo

Gut
A FasanoM M Levine

Abstract

Shigella enterotoxin 1 is a novel enterotoxin elaborated by Shigella flexneri 2a that causes fluid accumulation in rabbit ileal loops and a rise in short circuit current in Ussing chambers. To gain insights into the mechanism of action of shigella enterotoxin 1. Supernatants from genetically engineered clones either overexpressing shigella enterotoxin 1 or producing deletion mutants of the toxin were tested in rabbit ileum both in vitro and in vivo. In rabbit ileum shigella enterotoxin 1 induced an irreversible rise in short circuit current that was not mediated by any of the recognised intracellular mediators of secretion. Deletion of 90% of the A subunit of the holotoxin ablated its enterotoxicity. In the in vivo perfusion model, the toxin induced a time dependent decrease in water absorption, whereas no changes were detected in the segment perfused with supernatants obtained from the deletion mutant. Finally, partially purified toxin induced a dose dependent increment in short circuit current that reached its plateau at a toxin concentration of 4 x 10(-6) M. Shigella enterotoxin 1 induces a time and dose dependent intestinal secretion in the rabbit animal model, suggesting that it may be responsible for the watery phase of S...Continue Reading

Citations

Aug 31, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D BhattacharyaS Roy
Aug 12, 2014·Current Tropical Medicine Reports·Mussaret Bano Zaidi, Teresa Estrada-García
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·A T MaurelliA Fasano
Jun 25, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sofie LivioMyron M Levine
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Jan 23, 1999·Infection and Immunity·F R NoriegaM M Levine

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