Effect of short periods of sperm-oocyte coincubation during in vitro fertilization on embryo development in pigs

Theriogenology
M A GilE A Martinez

Abstract

The present study was conducted to determine if the efficiency of in vitro pig embryo production could be improved by a reduction in the period of time that oocytes are exposed to sperm during in vitro fertilization. A total of 1596 immature cumulus-oocyte complexes from five replicates were matured in vitro and inseminated with frozen-thawed spermatozoa (2000 spermatozoa/oocyte) for 10, 30, 60 min or 6h (control group). The oocytes from short coincubation times were washed three times in fertilization medium to remove spermatozoa not bound to the zona and transferred to another droplet of the same medium (containing no sperm) for 6h. After 6h, the oocytes from each group were cultured in embryo culture medium for another 6h to assess fertilization parameters and for 7 days to assess embryo development. After each period of coincubation, some oocytes were stained with Hoechst-33342 to count zona-bound sperm. Although the number of zona-bound sperm increased with the coincubation time (34.1 +/- 1.7, 46.8 +/- 2.8, 62.8 +/- 3.8 and 139.5 +/- 6.1 for 10, 30, 60 min and 6h, respectively, P < 0.02), the penetration rate was not significantly different among groups (61.3-68.2%). However, the efficiency of fertilization (number of mono...Continue Reading

References

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Citations

May 9, 2008·Reproduction, Fertility, and Development·C AlmiñanaE A Martinez
Nov 28, 2013·Theriogenology·Christopher G Grupen
Aug 5, 2008·Theriogenology·M A GilE A Martínez
Aug 19, 2007·Animal Reproduction Science·C AlmiñanaE A Martinez
Jun 20, 2008·Reproduction in Domestic Animals = Zuchthygiene·C AlmiñanaE A Martinez
May 28, 2011·Animal Science Journal = Nihon Chikusan Gakkaihō·Thanh Quang Dang-NguyenTakashi Nagai
Jul 16, 2010·Reproduction in Domestic Animals = Zuchthygiene·M A GilE A Martinez
Jul 16, 2010·Reproduction in Domestic Animals = Zuchthygiene·J N CaamañoE Gómez
Jul 16, 2015·Reproduction in Domestic Animals = Zuchthygiene·H Funahashi
May 24, 2011·Theriogenology·J N CaamañoE Gómez
Mar 18, 2017·The Journal of Reproduction and Development·Jenna L LoweChristopher G Grupen
Jan 18, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Sonja EntringerPathik D Wadhwa
Feb 25, 2014·Acta Biologica Hungarica·Melike Sapmaz-MetinC Uzal
Jun 20, 2014·Biology of Reproduction·Cui-Ling LuJie Qiao
Oct 10, 2014·Biology of Reproduction·Francesca LolicatoBarend M Gadella
Aug 2, 2019·Journal of Pineal Research·Jiyeon LeemJeong Su Oh
Jun 4, 2019·Reproduction in Domestic Animals = Zuchthygiene·Pengfei ShiFenghua Lu
Nov 26, 2019·Animal : an International Journal of Animal Bioscience·L B FerréP J Ross
May 2, 2019·Reproduction, Fertility, and Development·Jenna L LoweChristopher G Grupen
Nov 30, 2019·Reproduction, Fertility, and Development·A S Alcântara-NetoP Mermillod

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