Effect of short-term oral prednisone therapy on blood gene expression: a randomised controlled clinical trial

Respiratory Research
Hiroto TakiguchiDon D Sin

Abstract

Effects of systemic corticosteroids on blood gene expression are largely unknown. This study determined gene expression signature associated with short-term oral prednisone therapy in patients with chronic obstructive pulmonary disease (COPD) and its relationship to 1-year mortality following an acute exacerbation of COPD (AECOPD). Gene expression in whole blood was profiled using the Affymetrix Human Gene 1.1 ST microarray chips from two cohorts: 1) a prednisone cohort with 37 stable COPD patients randomly assigned to prednisone 30 mg/d + standard therapy for 4 days or standard therapy alone and 2) the Rapid Transition Program (RTP) cohort with 218 COPD patients who experienced AECOPD and were treated with systemic corticosteroids. All gene expression data were adjusted for the total number of white blood cells and their differential cell counts. In the prednisone cohort, 51 genes were differentially expressed between prednisone and standard therapy group at a false discovery rate of < 0.05. The top 3 genes with the largest fold-changes were KLRF1, GZMH and ADGRG1; and 21 genes were significantly enriched in immune system pathways including the natural killer cell mediated cytotoxicity. In the RTP cohort, 27 patients (12.4%) d...Continue Reading

References

Apr 10, 1997·The New England Journal of Medicine·P J Barnes, M Karin
Jul 3, 2002·Nature Reviews. Immunology·Michele Barry, R Chris Bleackley
Sep 10, 2002·Transplant Immunology·Derek MiddletonLynne Maxwell
Oct 3, 2002·The European Respiratory Journal·A Di StefanoI M Adcock
Dec 19, 2003·Nucleic Acids Research·Minoru KanehisaMasahiro Hattori
Jul 20, 2004·Journal of Cellular Biochemistry·Teresa Sanchez, Timothy Hla
Feb 3, 2006·The European Respiratory Journal·P J Barnes
Apr 22, 2008·Nature Immunology·Lewis L Lanier
Oct 17, 2008·Blood·Sabrina KuttruffAlexander Steinle
Jul 30, 2009·Therapeutic Advances in Respiratory Disease·David A NganDon D Sin
Jul 14, 2010·Journal of Medical Primatology·Peter ParhamLisbeth A Guethlein
May 23, 2013·JAMA : the Journal of the American Medical Association·Jörg D LeuppiJonas Rutishauser
Nov 22, 2016·Respirology : Official Journal of the Asian Pacific Society of Respirology·Jameel BarnawiRobyn Meech
Apr 30, 2017·Journal of Thoracic Disease·María-Teresa García-SanzFrancisco-Javier González-Barcala
Jan 20, 2018·The European Respiratory Journal·Surya P BhattMark T Dransfield
Apr 11, 2018·JAMA : the Journal of the American Medical Association·UNKNOWN US Burden of Disease CollaboratorsChristopher J L Murray

❮ Previous
Next ❯

Methods Mentioned

BETA
histone acetylation

Clinical Trials Mentioned

NCT02534402
NCT02050022

Software Mentioned

limma
WebGestalt
oligo

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

CFTR Mutant Structural Therapy

Over 1700 different mutations in the CFTR genes have been shown to cause cystic fibrosis. Here is the latest research on structural therapy for CFTR mutants.