Effect of Short-Term Stimulation with Interleukin-1β and Differentiation Medium on Human Mesenchymal Stromal Cell Paracrine Activity in Coculture with Osteoblasts

BioMed Research International
Jan O VossMartin J Stoddart

Abstract

Human mesenchymal stromal cells (hMSCs) exhibit the potential to accelerate bone healing by enhanced osteogenic differentiation. Interleukin-1β is highly expressed during fracture healing and has been demonstrated to exert a significant impact on the differentiation behaviour of hMSCs. Here, we investigate the effect of 2-hour IL-1β stimulation on the differentiation and paracrine activity of hMSCs in coculture with osteosarcoma cells in vitro. hMSCs from 3 donors were incubated for 2 hours with 10 ng/mL IL-1β and subsequently cocultured with MG63-GFP cells either in control or in differentiation medium in a transwell system for 28 days. Genetic and functional effects were investigated. hMSCs cultured in control medium exhibited a regulatory effect on cocultured MG63-GFP cells, resulting in upregulation of osteogenic gene expression in combination with increased ALP activity. However, while stimulated hMSCs cultured under differentiation conditions exhibit signs of osteogenic differentiation, osteogenic differentiation also caused an impaired regulatory effect on the cocultured MG63-GFP cells. Short stimulation of hMSCs has the potential to modify their long-term behaviour. In addition, undifferentiated hMSCs are able to regula...Continue Reading

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Citations

Mar 2, 2019·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·Ayça Koca TanrıverdiYaşar Murat Elçin
May 10, 2017·Journal of Tissue Engineering and Regenerative Medicine·Ugo D'AmoraLuigi Ambrosio
Feb 27, 2021·Drug Delivery and Translational Research·Angela CerusoRosa Maria Hernandez
Sep 8, 2019·Pediatric Research·Jose J Gil-CosanoFrancisco B Ortega

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Methods Mentioned

BETA
surgical resection
Reverse Transcription RNA
PCR

Software Mentioned

QuantStudio
GraphPad Prism

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