Effect of short-time povidone-iodine application on osteoblast proliferation and differentiation.

The Open Dentistry Journal
Patrick R SchmidlinF E Weber

Abstract

Povidone-iodine [polyvinylpyrrolidone-iodine complex (PVP-I)] is a broad-spectrum antimicrobial agent, frequently used in dentistry. In this study we investigated the short- and longterm effects on osteoblast number, viability, and function after short exposure to PVP-I with and without additional bone-morphogenetic protein-2 (BMP-2). Confluent osteoblast-like cell line (MC3T3-E1, subclone 24) cultures were exposed to pure PVP-I solution (7.7 mg/ml) and dilutions of 1:10, 1:100 and 1:1000 for 10 seconds and washed with phosphate buffer solution. Cell proliferation and viability was determined by MTT and differentiation status by alkaline phosphatase (ALP) activity 6 days after initial plating. In a separate experiment, long-term cell proliferation, viability and function were assessed 4 weeks after PVP-I treatment by MTT and deposited calcium using an Alizarin-red staining test. PVP-I decreased ALP activity substantially. Stimulation by BMP-2 recovered ALP activity to near control levels at 1:100 and 1:1000 dilutions of PVP-I. The MTT assay showed reduced proliferation of the preosteoblastic cells for all treatments, irrespective whether BMP-2 was used or not. Only at PVP-I dilutions of 1:1000 proliferation rate was back to nor...Continue Reading

References

Mar 1, 1985·The Journal of Hospital Infection·D J GockeW Pollack
Nov 1, 1988·Veterinary Surgery : VS·I R SanchezJ A McGuire
Jun 1, 1997·Periodontology 2000·R C Page, K S Kornman
Nov 1, 2001·Journal of Clinical Periodontology·B RoslingJ Lindhe
May 16, 2002·Dermatology : International Journal for Clinical and Investigative Dermatology·Tadashi Shiraishi, Yoshito Nakagawa
Oct 9, 2002·Journal of Periodontal Research·Jørgen Slots
May 20, 2003·Journal of Periodontal Research·T HoangJ Slots
Aug 17, 2005·Journal of Periodontology·Gary Greenstein
Feb 24, 2006·Dermatology : International Journal for Clinical and Investigative Dermatology·Akiko YoneyamaMuneo Hikida

❮ Previous
Next ❯

Citations

Feb 9, 2012·Current Reviews in Musculoskeletal Medicine·Ying LiDaniel Hedequist
Jul 1, 2011·Annals of Maxillofacial Surgery·K Senthil KumarRajesh Pandiarajan
Sep 2, 2011·Cell Biology International·Mauro MattioliBarbara Barboni
Feb 14, 2012·Archives of Oral Biology·Patrick R SchmidlinFranz E Weber
Mar 4, 2011·The Journal of Bone and Joint Surgery. American Volume·Jennifer BauerAllison Gilmore
Jun 8, 2017·Experimental and Therapeutic Medicine·Peng ZhangShiyi Chen
Nov 21, 2021·Journal of Materials Science. Materials in Medicine·Sarah J GarnerMichele E Barbour

❮ Previous
Next ❯

Software Mentioned

SSPE

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]
Arthur K Balin, Loretta Pratt
International Journal of Oral and Maxillofacial Surgery
Gururaj Arakeri, Peter A Brennan
Journal of Applied Oral Science : Revista FOB
Erica Del Peloso RibeiroMárcio Zaffalon Casati
© 2022 Meta ULC. All rights reserved