Abstract
Sideritis leptoclada O. Schwarz et P.H. Davis extracts were evaluated for its singlet oxygen production capacity using spectrophotometric method. The extracts producing singlet oxygen were then evaluated for cytotoxicity against malignant melanoma cancer (HT-144) and fibroblast (3T3) cell lines using the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay. The photocytotoxicity against the HT-144 human melanoma cell line in the presence of illumination (∼≥400 nm) was also evaluated. In the standard MTT assay, the ethanol extract of S. leptoclada (100 µg/ml) showed 83.49±3.33% inhibition of HT-144 cancer cells, whereas in the illuminated MTT assay, it showed 77.46±1.97% inhibition of HT-144 cancer cells. The effects of ethanol extract on reactive oxygen species production, apoptosis, and tumor necrosis factor-α secretion were also evaluated on HT-144 cell lines. The extract triggered an increase in intracellular reactive oxygen species production and tumor necrosis factor-α secretion compared with the respective controls. Thus, the ethanol extract may cause apoptosis. The LC-MS/MS analyses of S. leptoclada ethanolic extract showed that it has quinic acid (137213±11.25 µg/g extract), malic acid (1468±0.16 µ...Continue Reading
References
Nov 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J W Larrick, S C Wright
Sep 1, 1989·Journal of Natural Products·M J AlcarazA Villar
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Jul 17, 1995·Journal of Immunological Methods·I VermesC Reutelingsperger
Mar 22, 2000·Biochemical Systematics and Ecology·M L BondìM S Simmonds
Jun 6, 2003·Oncogene·María S Soengas, Scott W Lowe
Feb 12, 2005·Antioxidants & Redox Signaling·Mehdi ShakibaeiBharat B Aggarwal
Feb 22, 2005·Lancet·John F ThompsonRichard F Kefford
Nov 22, 2005·Journal of Biochemical and Biophysical Methods·Ana GomesJosé L F C Lima
Mar 25, 2006·Phytotherapy Research : PTR·E LoğoğluI Sakõyan
Jul 29, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Han-Ming Shen, Shazib Pervaiz
Apr 9, 2009·Virchows Archiv : an International Journal of Pathology·Anna BatistatouUNKNOWN ESP Dermatopathology Working Group
Mar 6, 2010·Cell Death and Differentiation·J J KimY D Yoo
Mar 17, 2010·Archives of Dermatology·Howard W RogersBrett M Coldiron
May 14, 2010·PloS One·Yasuhiro Matsuo, Tetsu Kamitani
Jun 30, 2010·Journal of Enzyme Inhibition and Medicinal Chemistry·M Iqbal ChoudharyFatima Z Basha
Mar 23, 2011·Journal of Ethnopharmacology·E González-BurgosM P Gómez-Serranillos
Oct 26, 2011·Fitoterapia·Hasan KirmizibekmezSonia Piacente
Jan 26, 2012·Planta medica·Vanja M TadićIvana Arsic
Oct 5, 2012·Development·Nousheen IramAdelheid Elbe-Bürger
May 7, 2013·Phytochemistry·E González-BurgosM P Gómez-Serranillos
May 29, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Dimitra A A KogiannouAndriana C Kaliora
Sep 28, 2013·Planta medica·Ivica JeremicAleksandra Isakovic
Jan 16, 2016·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Max DisseSarah S Schram
Citations
Jan 30, 2019·Molecular Biology Reports·Fatma Aydoğmuş-ÖztürkMuhammad Iqbal Choudhary
Jul 28, 2020·Molecular Biology Reports·Fatma Aydoğmuş-ÖztürkMuhammad Iqbal Choudhary
Oct 21, 2020·Biomolecules & Therapeutics·Min-Ji RyuSang-Jip Nam