Effect of silencing NEK2 on biological behaviors of HepG2 in human hepatoma cells and MAPK signal pathway

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Mei-Xia ZhangZhi-Xiong Long

Abstract

To investigate the expression level of NEK2 in 40 tissue specimens of primary liver cancer and to search for clues whether the effect of NEK2 depletion plays a role on biological behaviors of HepG2 cells and the relevant molecular mechanism are the objectives of this study. Real-time PCR and immunohistochemistry assessed expression level of NEK2 in specimens of cancerous tissues and carcinoma-adjacent tissues. The NEK2 expression level in HepG2, Huh7, SMMC, and 7402 cells was detected by real-time PCR and western blot to screen experimental cell line. To assess the expression levels of NEK2 mRNA and protein, an effective siRNA transfected into the HepG2 cells was designed. CCK8 and colony-forming assays were performed to verify short-term and long-term proliferative activities, respectively. Capacity of apoptosis and cell cycle changes were assessed by flow cytometry. Ability of transference and invasion was measured by Transwell Chambers. Western blot approach was used to determine the protein expression levels. There was significantly high expression level of NEK2 in cancerous tissues compared to adjacent tissues. The expression of NEK2 was higher in HepG2 cells than other cell lines. Real-time PCR and western blot shown ther...Continue Reading

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Citations

Dec 25, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhan ShiXiaoqing Sun
Dec 8, 2016·International Journal of Cancer. Journal International Du Cancer·Sheng-Ming WuKwang-Huei Lin
Mar 30, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiao-Qin HeXi-Ming Xu
Jun 18, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yuanyuan GanXiming Xu
Jul 19, 2018·ESMO Open·Peter A van DamSteven Van Laere
Aug 2, 2017·Cancer Biomarkers : Section a of Disease Markers·Luoqin FuGuoqing Ru

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