Effect of silica-coated magnetic nanoparticles on rigidity sensing of human embryonic kidney cells.

Journal of Nanobiotechnology
Abdurazak Aman KeteboSungsu Park

Abstract

Nanoparticles (NPs) can enter cells and cause cellular dysfunction. For example, reactive oxygen species generated by NPs can damage the cytoskeleton and impair cellular adhesion properties. Previously, we reported that cell spreading and protrusion structures such as lamellipodia and filopodia was reduced when cells are treated with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate (MNPs@SiO2(RITC)), even at 0.1 μg/μL. These protruded structures are involved in a cell's rigidity sensing, but how these NPs affect rigidity sensing is unknown. Here, we report that the rigidity sensing of human embryonic kidney (HEK293) cells was impaired even at 0.1 μg/μL of MNPs@SiO2(RITC). At this concentration, cells were unable to discern the stiffness difference between soft (5 kPa) and rigid (2 MPa) flat surfaces. The impairment of rigidity sensing was further supported by observing the disappearance of locally contracted elastomeric submicron pillars (900 nm in diameter, 2 μm in height, 24.21 nN/μm in stiffness k) under MNPs@SiO2(RITC) treated cells. A decrease in the phosphorylation of paxillin, which is involved in focal adhesion dynamics, may cause cells to be insensitive to stiffness differences when they ar...Continue Reading

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Citations

Jan 13, 2021·Journal of Nanobiotechnology·Tae Hwan ShinGwang Lee
Aug 14, 2021·Particle and Fibre Toxicology·Tae Hwan ShinGwang Lee
Aug 29, 2021·Pharmaceutics·Elena K Schneider-Futschik, Felisa Reyes-Ortega
Sep 15, 2021·Journal of Applied Biomaterials & Functional Materials·Chan Woo KimKwan Soo Hong

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Methods Mentioned

BETA
biosensors
Fluorescence
X-ray

Software Mentioned

ImageJ
FIJI ImageJ
Pillar Tracker
FiloQuant

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