Effect of silver nanoparticles on Pseudomonas putida biofilms at different stages of maturity

Journal of Hazardous Materials
Pumis ThuptimdangEakalak Khan

Abstract

This study determined the effect of silver nanoparticles (AgNPs) on Pseudomonas putida KT2440 biofilms at different stages of maturity. Three biofilm stages (1-3, representing early to late stages of development) were identified from bacterial adenosine triphosphate (ATP) activity under static (96-well plate) and dynamic conditions (Center for Disease Control and Prevention biofilm reactor). Extracellular polymeric substance (EPS) levels, measured using crystal violet and total carbohydrate assays, and expression of the EPS-associated genes, csgA and alg8, supported the conclusion that biofilms at later stages were older than those at earlier stages. More mature biofilms (stages 2 and 3) showed little to no reduction in ATP activity following exposure to AgNPs. In contrast, the same treatment reduced ATP activity by more than 90% in the less mature stage 1 biofilms. Regardless of maturity, biofilms with EPS stripped off were more susceptible to AgNPs than controls with intact EPS, demonstrating that EPS is critical for biofilm tolerance of AgNPs. The findings from this study show that stage of maturity is an important factor to consider when studying effect of AgNPs on biofilms.

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Citations

Jun 1, 2016·Journal of Hazardous Materials·Katia Noguera-Oviedo, Diana S Aga
Jun 11, 2016·International Wound Journal·Silvestre Ortega-PeñaEdgar Krötzsch
Jul 21, 2016·Environmental Science & Technology·Connie Walden, Wen Zhang
Aug 12, 2016·Nature Reviews. Microbiology·Hans-Curt FlemmingStaffan Kjelleberg
Jun 13, 2019·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Hossein JamalifarMohammad Reza Fazeli
Nov 11, 2017·Environmental Science. Nano·Sijie LinPu Chun Ke
Jun 30, 2019·Environmental Science and Pollution Research International·Hussam FallatahKonstantinos Gkatzionis
Feb 12, 2020·Water Research·Hans-Curt Flemming

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