Effect of Simulated Microgravity Conditions on Differentiation of Adipose Derived Stem Cells towards Fibroblasts Using Connective Tissue Growth Factor

Iranian Journal of Biotechnology
Farid EbnerasulyMojtaba Darbouy

Abstract

Background: Mesenchymal stem cells (MSCs) are multipotent cells able to differentiating into a variety of mesenchymal tissues including osteoblasts, adipocytes and several other tissues. Objectives: Differentiation of MSCs into fibroblast cells in vitro is an attractive strategy to achieve fibroblast cell and use them for purposes such as regeneration medicine. The goal of this study was investigate the simulated microgravity effect on differentiation of Adipose Derived Stem Cells (ADSCs) to fibroblasts. Materials and Methods: To fibroblast differentiation 100 ng.mL-1 of connective tissue growth factor (CTGF), and for simulation microgravity, 2D clinostat was used. After isolation the human ADSCs from adipose, cells were passaged, and at passages 3 they were used for characterization and subsequent steps. After 7 days of CTGF and simulated microgravity treatment, proliferation, and differentiation were analyzed collectively by MTT assay, quantitative PCR analyses, and Immunocytochemistry staining. Results: MTT assay revealed that CTGF stimulate the proliferation but simulated microgravity didn't have statistically significant effect on cell proliferation. In RNA level the expression of these genes are investigated: collagen typ...Continue Reading

References

Sep 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A I Caplan
Dec 1, 1989·Experimental Cell Research·C LingeR F Brooks
Jun 1, 1984·Journal of Speech and Hearing Research·A L PerlmanD S Cooper
Aug 1, 1995·The International Journal of Biochemistry & Cell Biology·C M Kielty, C A Shuttleworth
Jul 1, 1995·The Journal of Cell Biology·F StrutzE G Neilson
Aug 13, 1998·Nature Medicine·B R Unsworth, P I Lelkes
Mar 27, 1999·Seminars in Cancer Biology·M López-BotetM Llano
Apr 15, 1999·Nature Medicine·T G HammondJ H Kaysen
Sep 26, 2000·Molecular Genetics and Metabolism·E E Moussad, D R Brigstock
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J VassyD Schoevaert
Jan 1, 1994·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·E L Kordyum
Jan 1, 1983·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·M G TairbekovF V Sushkov
Jan 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gregory H AltmanDavid L Kaplan
Mar 29, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniela GrimmAugusto Cogoli
May 7, 2002·Cell Biology International·Yoshiko AraseNobuo Suzuki
Sep 24, 2002·Journal of Cell Science·Roumen Pankov, Kenneth M Yamada
Sep 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Howard Y ChangPatrick O Brown
Nov 13, 2002·Arthritis and Rheumatism·Eugene Y KissinRobert Lafyatis
Nov 19, 2004·Nature·Neil A BhowmickHarold L Moses
Apr 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gary R Grotendorst, Matthew R Duncan
Feb 16, 2006·The International Journal of Developmental Biology·Susan J Crawford-Young
Aug 29, 2007·Investigative Ophthalmology & Visual Science·Xiaoqing GuoJeffrey W Ruberti
Nov 8, 2007·Cell Motility and the Cytoskeleton·W A LoesbergJ A Jansen
Apr 10, 2008·Cell Stem Cell·Paolo BiancoPaul J Simmons
Apr 12, 2008·Proceedings of the American Thoracic Society·Sem H Phan
Jun 19, 2008·Experimental Dermatology·Katrin LorenzAugustinus Bader
Feb 20, 2009·Tissue Engineering. Part a·Daniela GrimmJohann Bauer
Jan 18, 2012·Journal of Burn Care & Research : Official Publication of the American Burn Association·Mohammad Ali BaharAziz Ghahary

❮ Previous
Next ❯

Citations

Nov 20, 2019·The Journal of International Medical Research·Su WangXiaoyu Wang
May 1, 2020·Stem Cells Translational Medicine·Daniela GrimmMarcus Krüger
Mar 17, 2021·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Aifeng ChenXu'ai Lin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.