Effect of single point mutations in citrate synthase on binding to GroEL

FEBS Letters
R ZahnA Plückthun

Abstract

Six single point mutants of yeast citrate synthase were analyzed for binding to the molecular chaperone GroEL. In contrast to the wild-type and G276S, all other G276-mutants were able to displace pre-beta-lactamase from GroEL. The off-rate constant for pre-beta-lactamase must be at least partially rate-limiting, leading to an equilibrium dissociation constant between 10(-10) M and 10(-12)M. Direct evidence for binding of citrate synthase was obtained from gel filtration experiments. The results suggest that thermodynamic rather than structural features of the mutants determine the degree of binding to the chaperone.

References

Nov 4, 1991·FEBS Letters·T E Gray, A R Fersht
Jan 2, 1992·Nature·M J Gething, J Sambrook
Jan 1, 1986·Annual Review of Biophysics and Biophysical Chemistry·G Wiegand, S J Remington
Nov 7, 1995·Biochemistry·L S ItzhakiA R Fersht
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·F J Corrales, A R Fersht
Jan 20, 1995·The Journal of Biological Chemistry·Z LinE Eisenstein
Jan 1, 1994·Trends in Biochemical Sciences·F U HartlT Langer
May 1, 1994·Journal of Structural Biology·J R HarrisR Zahn
Feb 5, 1993·Journal of Molecular Biology·R ZahnW Baumeister
Aug 20, 1993·Journal of Molecular Biology·T E Gray, A R Fersht
May 10, 1996·Science·U von Zahn, D M Hunten
May 1, 1993·Current Biology : CB·H R SaibilR J Ellis

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Citations

Apr 1, 1997·Protein Science : a Publication of the Protein Society·W A Fenton, A L Horwich
Oct 1, 1996·Current Opinion in Biotechnology·C Kurland, J Gallant
Jun 8, 2012·International Journal of Endocrinology·Sumantha BhattShiguang Qian
Sep 27, 2000·Cell Stress & Chaperones·M D de BeusC M Teschke
Jun 27, 1997·Journal of Molecular Biology·S PerrettA R Fersht
Nov 6, 2009·Journal of Proteome Research·Kuvat T MomynalievVadim M Govorun

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