Effect of siRNA-mediated silencing of Bmi-1 gene expression on HeLa cells

Cancer Science
Yue JiangHong Yang

Abstract

B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi-1) is highly expressed in several malignant tumors and its expression level is positively correlated with tumor invasion, distant metastasis, prognosis, and recurrence. In the present study, the biological effect of small interfering RNA (siRNA) that targeted Bmi-1 expression was studied in human cervical carcinoma cell line HeLa cells. Bmi-1 siRNA inhibited the expression of Bmi-1 at the mRNA and protein levels in HeLa cells, which significantly affected proliferation, colony formation, and migration of HeLa cells in vitro and in vivo. Therefore, silencing Bmi-1 may be a potential therapeutic option for the management of some human cancers.

References

Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·N J CaplenR A Morgan
Nov 2, 2001·Nature·T ReyaI L Weissman
Nov 25, 2003·Nature·Titia Sijen, Ronald H A Plasterk
Sep 30, 2004·The Breast : Official Journal of the European Society of Mastology·Joo Heon KimJae Wha Kim
Mar 15, 2006·Current Medicinal Chemistry·E GalmozziC A M La Porta
May 11, 2006·Biochemical and Biophysical Research Communications·Huimin ZhouJianing Zhang
Sep 23, 2006·The New England Journal of Medicine·Craig T JordanMark Noble
Mar 30, 2007·The American Journal of Pathology·Hongjuan CuiHan-Fei Ding
Jun 29, 2007·International Journal of Cancer. Journal International Du Cancer·Daniela Mihic-ProbstHolger Moch

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Citations

Nov 30, 2011·Epigenomics·Koji Itahana, Goberdhan P Dimri
Mar 17, 2012·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Xiuxiang MengHong Yang
Sep 21, 2011·Mutation Research·Itzel Sifuentes-RomeroAlejandra García-Gasca
Apr 13, 2011·Oral Oncology·Hui ChenJie Tian
Aug 23, 2011·International Journal of Cancer. Journal International Du Cancer·Xin ZhangZhaogang Dong
Jan 13, 2017·Molecular Oncology·Lobna ElkhadragyWeiwen Long

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