Effect of smoking on MAP kinase-induced modulation of IL-8 in human alveolar macrophages

The European Respiratory Journal
Andrea KochK F Chung

Abstract

Inflammatory cytokine production by alveolar macrophages (AMs) is regulated by transcriptional activation and may be increased by cigarette smoking. The smoking-induced regulation of interleukin (IL)-8 by extracellular signal-regulated kinase (ERK)-1 and -2, p38 mitogen-activated protein kinase (MAPK) and the transcription factor nuclear factor-kappaB (NF-kappaB) in lipopolysaccharide-stimulated AMs was assessed in nine smokers compared with nine healthy nonsmokers. IL-8 production was dependent on phosphorylation of ERK-1 and -2 and p38 MAPK, as examined by PD 098059 (10 microM), an inhibitor of the upstream activator of MAPK kinase (MKK)-1, and SB 203580 (10 microM), an inhibitor of p38 MAPK. IL-8 release and the inhibitory effect of PD 098059 were increased in AMs from smokers. Moreover, ERK-2 messenger ribonucleic acid expression, as examined by reverse transcriptase polymerase chain reaction and phosphorylation of ERK-2 using Western blots, were increased in AMs from smokers, indicating a smoking-induced modulatory role of ERK-1 and -2. Lipopolysaccharide-induced IL-8 production was dependent on activation of NF-kappaB, as examined by SN 50 (100 microM), an inhibitor of NF-kappaB translocation, and the specific NF-kappaB i...Continue Reading

Citations

Mar 8, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Adriana HeguyRonald G Crystal
Dec 15, 2005·The Journal of Experimental Medicine·Clifford C TaggartNoel G McElvaney
Jun 16, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Zhenjun LiRavi Mahadeva
Apr 15, 2006·American Journal of Respiratory and Critical Care Medicine·Mark HewKian Fan Chung
Jun 11, 2011·International Journal of Chronic Obstructive Pulmonary Disease·Rahul G Sangani, Andrew J Ghio
Aug 10, 2010·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·A Garcia-RiosJ M Ordovas
Apr 11, 2009·The American Journal of Pathology·Antje PrasseAndreas C Renkl
Jun 17, 2006·Biochimica Et Biophysica Acta·Loutfig DemirjianVincent Duronio
Oct 13, 2005·Journal of Cardiovascular Pharmacology·Liangqi XieXian Wang
May 31, 2013·The Journal of Pharmacology and Experimental Therapeutics·Jürgen KnoblochAndrea Koch
Oct 9, 2007·The Journal of Pharmacology and Experimental Therapeutics·Susan J TudhopeLouise E Donnelly
Dec 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew J ThorleyTeresa D Tetley
May 15, 2021·Thorax·Sebastian T LuggDavid R Thickett

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.