Effect of sodium benzoate preservative on micronucleus induction, chromosome break, and Ala40Thr superoxide dismutase gene mutation in lymphocytes

BioMed Research International
Malinee Pongsavee

Abstract

Sodium benzoate is food preservative that inhibits microbial growth. The effects of sodium benzoate preservative on micronucleus induction, chromosome break, and Ala40Thr superoxide dismutase gene mutation in lymphocytes were studied. Sodium benzoate concentrations of 0.5, 1.0, 1.5, and 2.0 mg/mL were treated in lymphocyte cell line for 24 and 48 hrs, respectively. Micronucleus test, standard chromosome culture technique, PCR, and automated sequencing technique were done to detect micronucleus, chromosome break, and gene mutation. The results showed that, at 24- and 48-hour. incubation time, sodium benzoate concentrations of 1.0, 1.5, and 2.0 mg/mL increased micronucleus formation when comparing with the control group (P < 0.05). At 24- and 48-hour. incubation time, sodium benzoate concentrations of 2.0 mg/mL increased chromosome break when comparing with the control group (P < 0.05). Sodium benzoate did not cause Ala40Thr (GCG→ACG) in superoxide dismutase gene. Sodium benzoate had the mutagenic and cytotoxic toxicity in lymphocytes caused by micronucleus formation and chromosome break.

References

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Citations

Mar 23, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Betul Yilmaz, Arzu Zeynep Karabay
Dec 13, 2017·EFSA Journal·UNKNOWN EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP)Secundino López Puente
Dec 16, 2017·Journal of Biochemical and Molecular Toxicology·Mohammad Javad KhoshnoudMarzieh Rashedinia
Aug 30, 2018·Nature Communications·He HuangYingming Zhao
Jul 28, 2020·Frontiers in Microbiology·Nur Fadhilah Khairil MokhtarRaha Abdul Rahim
Jun 19, 2021·Toxicology Research·Anthony Tope OlofinnadeOlugbenga Adekunle Olowe

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