Effect of Sodium Fluoride on the Proliferation and Gene Differential Expression in Human RPMI8226 Cells

Biological Trace Element Research
Hong HeZhou Zhou

Abstract

Although fluoride is known to reduce the incidence of caries, chronic excessive fluoride exposure can impair human health, even resulting in fluorosis. Now the underlying mechanisms of fluoride-induced toxicity are not fully understood. So, we conducted this study with the purpose of investigating the effect of sodium fluoride (NaF) in human RPMI8226 cells. In this experiment, human RPMI8226 cells were cultured with varied doses of fluoride (10, 20, 40, 80, 160, 320 μM). After 48 h exposure, the change of cell viability was examined by CCK-8 assay, and also the messenger RNA (mRNA) expression of relevant genes was assessed by QRT-PCR. Compared to the control group, fluoride exposure increased the human RPMI8226 cells viability at relatively lower levels (10-160 μM); however, when the concentration reached to 320 μM, the cell proliferation was significantly inhibited (p < 0.05). In addition, the genes mRNA expression, including ANKRD1, CRSP6, KLF2, SBNO2, ZNF649, FANCM, PDGFA, RNF152, CDK10, and CETN2 changed in a concentration-dependent manner and increased with fluoride exposure concentration. The results suggest that overexposure to fluoride (160-320 μM) can induce cytotoxicity and regulate relevant genes expression. Our find...Continue Reading

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Citations

Nov 26, 2015·Journal of Pharmacological and Toxicological Methods·Miroslav RadenkovićMilica Prostran
Jul 23, 2019·International Journal of Radiation Biology·Magdalena Kimsa-DudekKatarzyna Pawłowska-Góral
Aug 10, 2020·Biological Trace Element Research·Sedat ÇetinVeysel Yüksek
Aug 20, 2021·Ecotoxicology and Environmental Safety·Zhuanxu OuyangHui Zhang
Sep 28, 2021·Biological Trace Element Research·Rıskiye KorkmazSemiha Dede

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