PMID: 7087464Mar 1, 1982Paper

Effect of sodium molybdate on cytosolic estrogen receptor

Journal of Steroid Biochemistry
B MoncharmontP Cuatrecasas

Abstract

Estrogen binding activity of crude calf uterus cytosol is rapidly destroyed in heating. The time course of inactivation at 37 degrees C shows a biphasic pattern; sodium molybdate (5-10 mM) completely blocks one of the components in the estradiol-free cytosol, while it has little effect on cytosolic receptor complexed with estradiol. Partially purified native 8S receptor loses its heat sensitivity, and, as a consequence, the molybdate effect disappears. By sucrose gradient analysis of crude cytosol it is evident that molybdate does not affect the sedimentation properties of the estradiol receptor at low temperature. However, at increasing temperatures, molybdate prevents the disappearance of the receptor peak in the crude cytosol or the formation of large, KCl-resistant, aggregates in the presence of estradiol. The partially purified native 8S receptor does not aggregate on heating; addition to it of receptor-depleted cytosol results in the recovery of heat inactivation and aggregate formation, and this is prevented by molybdate. Molybdate has no protective effect on any other inactivating agent which does not act through aggregation of receptors. A crude cytosolic preparation of the receptor which is unable to form heat-depende...Continue Reading

References

Jan 26, 1976·Biochemical and Biophysical Research Communications·R M Roberts, F W Bazer
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C J NielsenW B Pratt
Apr 1, 1972·Journal of Steroid Biochemistry·E V JensenE R DeSombre
Nov 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·S G Korenman, B R Rao
Jul 26, 1968·Biochemical and Biophysical Research Communications·T Erdos
Apr 1, 1969·Steroids·E V JensenE R DeSombre
Jan 1, 1980·Journal of Steroid Biochemistry·W W GrodyB W O'Malley
Jul 1, 1980·The Journal of Cell Biology·J E Heuser, M W Kirschner
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M SchliwaK R Porter
Jan 8, 1980·Biochemistry·H Nishigori, D Toft
Jan 1, 1980·Journal of Steroid Biochemistry·G A PucaV Sica

Citations

Mar 1, 1984·Journal of Steroid Biochemistry·S L SchneiderA A Sandberg
Jun 1, 1984·Journal of Steroid Biochemistry·M J Weinberger
Sep 1, 1986·Journal of Steroid Biochemistry·W J Hendry, B J Danzo
Apr 30, 1985·Biochemical and Biophysical Research Communications·A M MolinariG A Puca
Dec 1, 1988·European Journal of Cancer & Clinical Oncology·J P van NettenC Fletcher

Related Concepts

Bos indicus
Cytoplasmic Matrix
Estradiol, (16 alpha,17 beta)-Isomer
Hydrogen-Ion Concentration
Molybdenum
Osmolality
Slow-K
Protein Denaturation
Estrogen Nuclear Receptor
Uterus

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