PMID: 6538161Feb 1, 1984Paper

Effect of some phthalate esters and other testicular toxins on primary cultures of testicular cells

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
T J Gray, J A Beamand

Abstract

Mixed cultures of Sertoli and germ cells were prepared from rat testes and their response to some model testicular toxins was studied. Cultures consisted of a monolayer of Sertoli cells to which clusters of spermatocytes and spermatogonia adhered. With time in culture, germ cells progressively detached from the Sertoli cells into the medium. Addition of mono-(2-ethylhexyl) phthalate (MEHP) to the culture medium resulted in a concentration-dependent increase in the rate of germ-cell detachment over the range 10(-7) - 10(-4) M. No such effect was produced by di-(2-ethylhexyl) phthalate or 2-ethylhexanol. An increased rate of germ-cell detachment was also produced by other phthalate monoesters known to cause testicular damage in vivo, whereas similar concentrations of a number of monophthalates not known to affect the testis in vivo had no such effect on the cultures. Known age and species differences in the testicular toxicity of di-(2-ethylhexyl) phthalate could be reproduced in cultures treated with MEHP. There was little effect on the viability of either germ cells or Sertoli cells at concentrations of MEHP that caused marked germ-cell detachment, but there were changes in Sertoli-cell morphology. Increased germ-cell detachmen...Continue Reading

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Citations

Jan 9, 2001·American Journal of Industrial Medicine·J A TicknerM Rossi
Jul 5, 2005·Archives of Toxicology·Manori J SilvaAntonia M Calafat
Jan 29, 2013·Environmental Science and Pollution Research International·Edith GuibertPascal Froment
Jun 1, 1986·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T J Gray
Sep 18, 1996·Molecular and Cellular Endocrinology·P GuillaumotM Benahmed
Jan 1, 1987·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P M FosterM S Prout
Jan 1, 1989·Toxicology in Vitro : an International Journal Published in Association with BIBRA·N R WorrellT J Gray
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R E Chapin, J Phelps
Jan 1, 1993·Reproductive Toxicology·C F Colie
Jul 1, 1993·Reproductive Toxicology·W W KuR E Chapin
Jan 1, 1993·Reproductive Toxicology·J C Lamb, R E Chapin
Jan 1, 1993·Reproductive Toxicology·A Steinberger, G Klinefelter
Apr 29, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Antonia M CalafatLarry L Needham
Apr 15, 2004·Reproductive Toxicology·Céline FioriniGeorges Pointis
Jun 22, 1999·Molecular and Cellular Endocrinology·P Guillaumot, M Benahmed
Jul 25, 2000·Toxicology Letters·N AdhikariD K Saxena
Sep 25, 2002·Toxicology Letters·John H RichburgDavid J Dix
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C PineauB Jégou
Apr 12, 2001·Reproductive Toxicology·J H DeesV Papadopoulos
Mar 19, 2003·Reproductive Toxicology·Ken L TakahashiShoji Teramoto
Aug 24, 1999·Reproductive Toxicology·N SinhaD K Saxena

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