Effect of sorbitol and glycerol on the stability of trypsin and difference between their stabilization effects in the various solvents

Biotechnology and Applied Biochemistry
Mohammad PazhangNader Chaparzadeh

Abstract

The effect of glycerol and sorbitol on the stability of porcine pancreas trypsin was investigated in this work. Molecular dynamics simulation and thermostability results showed that trypsin has two flexible regions, and polyols (sorbitol and glycerol) stabilize the enzyme by decreasing the flexibility of these regions. Radial distribution function results exhibited that sorbitol and glycerol were excluded from the first water layer of the enzyme, therefore decrease the flexibility of the regions by preferential exclusion. Also, results showed that the stabilization effect of sorbitol is more than glycerol. This observation could be because of the larger decrease in the fluctuations of trypsin in the presence of sorbitol. We also examined the role of solvent's hydrophobicity in enzyme stabilization by sorbitol and glycerol. To do so, the thermostability of trypsin was evaluated in the presence of solvents with different hydrophobicity (methanol, ethanol, isopropanol and n-propanol) in addition to the polyols. Our results depicted that glycerol is a better stabilizer than sorbitol in the presence of hydrophobic solvents (n-propanol), whereas sorbitol is a better stabilizer than glycerol in the presence of hydrophilic solvents (me...Continue Reading

References

Feb 1, 1991·International Journal of Peptide and Protein Research·M T Miranda, M Tominaga
Sep 27, 1995·Biochimica Et Biophysica Acta·C O Fágáin
Mar 1, 1993·Biotechnology Progress·Y ItoY Imanishi
Jan 1, 1994·Methods in Enzymology·N D Rawlings, A J Barrett
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·R V Rariy, A M Klibanov
Jun 7, 2000·Bioscience Reports·J Rodakiewicz-NowakL A Golovleva
Jun 27, 2000·Journal of Biotechnology·R Jaenicke
Feb 13, 2001·Biochemical and Biophysical Research Communications·L M SimonI Laczkó
Feb 24, 2001·Nature·A M Klibanov
Oct 27, 2001·Acta Crystallographica. Section D, Biological Crystallography·S DeepthiV Pattabhi
Dec 18, 2001·Proteins·Darin M Taverna, Richard A Goldstein
Dec 26, 2001·Current Opinion in Structural Biology·C Mattos, D Ringe
Feb 13, 2002·Structure·Claus A F AndersenBurkhard Rost
Jul 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Serge N Timasheff
Oct 29, 2002·Critical Reviews in Food Science and Nutrition·David Julian McClements
Feb 21, 2003·Biotechnology and Applied Biochemistry·Reynaldo VillalongaRaffaele Porta
Mar 23, 2004·Biotechnology and Applied Biochemistry·Ipsita Roy, Munishwar Nath Gupta
Mar 27, 2004·Protein Science : a Publication of the Protein Society·Hanna-Kirsti Schrøder LeirosArne O Smalås
Jun 23, 2004·European Journal of Biochemistry·Munishwar N Gupta, Ipsita Roy
Jul 21, 2004·Journal of Computational Chemistry·Chris OostenbrinkWilfred F van Gunsteren
Jul 24, 2004·Acta Crystallographica. Section D, Biological Crystallography·Alexander W Schüttelkopf, Daan M F van Aalten
Aug 20, 2004·Biotechnology and Applied Biochemistry·Maria L VillalongaReynaldo Villalonga
Jul 14, 2007·International Journal of Biological Macromolecules·H A SathishV Prakash
Oct 13, 2009·Biochemistry·Vincent VagenendeBernhardt L Trout
Jun 2, 2010·Protein Engineering, Design & Selection : PEDS·S Mohsen AsghariKhosro Khajeh
Jan 26, 2011·The Journal of Chemical Physics·Faramarz MehrnejadFarahnoosh Doustdar
Jul 16, 2011·Advanced Drug Delivery Reviews·Satoshi OhtakeTsutomu Arakawa

❮ Previous
Next ❯

Citations

Dec 18, 2018·Physical Chemistry Chemical Physics : PCCP·Vikas Dubey, Snehasis Daschakraborty
Sep 25, 2019·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Adama NdaoJose Valéro
Jul 14, 2019·Applied Biochemistry and Biotechnology·Nelson LibardiLuciana Porto de Souza Vandenberghe
Dec 27, 2019·Biophysical Chemistry·Kirthi Joshi, Abani K Bhuyan
Aug 30, 2021·Biotechnology Advances·Rafael C RodriguesRoberto Fernandez-Lafuente

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.