Effect of SOST gene deletion on the progression of renal interstitial fibrosis in obstructive kidney injury

Renal Failure
Lu-Fei WangDing Bai

Abstract

The role of SOST/sclerostin in mediating tissue fibrogenic response to injury/inflammation remains largely unknown. Thus, we conducted this study to determine whether SOST/sclerostin plays a role in renal interstitial fibrosis (RIF) for the first time. Unilateral ureteral obstruction (UUO) was performed to create obstructive kidney injury model. Twelvemale SOST knockout (SOST KO) mice and 12 age-matched wild-type (WT) mice were divided into three groups: sham surgery, UUO 3 d and UUO 7 d. The mice were sacrificed at each time point and kidney tissues were collected. Histopathological changes were evaluated by hematoxylin and eosin and Masson staining, while α-smooth muscle actin (α-SMA), type I collagen (Col-I) and fibronectin (FN) expression levels were detected by RT-PCR and western-blot. In sham control group, neither WT nor SOST KO exhibited fibrotic change. On 3 days after UUO, total renal histopathological score and fibrotic area were aggravated and α-SMA, Col-I and FN expressions were upregulated, but no difference was observed between WT and SOST KO. On 7 days after UUO, compared with WT, SOST KO mice showed higher total renal histopathological score and fibrotic area percentage, as well as a higher level of fibrogenic ...Continue Reading

References

Jun 23, 1988·The New England Journal of Medicine·S KlahrI Ichikawa
Mar 22, 2005·The Journal of Biological Chemistry·Xiaofeng LiDianqing Wu
May 24, 2005·The Journal of Biological Chemistry·Mikhail SemënovXi He
May 10, 2006·Advances in Immunology·Mark L Lupher, W Michael Gallatin
Feb 14, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xiaodong LiChris Paszty
Feb 19, 2008·Bone·Lynda F Bonewald, Mark L Johnson
Apr 9, 2008·Contributions to Nephrology·Yoshitaka IsakaEnyu Imai
Oct 28, 2008·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Jon J SnyderAllan J Collins
Apr 3, 2009·Kidney International·Robert L ChevalierBarbara A Thornhill
Jul 15, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ina KramerMichaela Kneissel
Sep 15, 2010·Nature Reviews. Nephrology·Peter BoorJürgen Floege
Jun 1, 2011·Expert Opinion on Therapeutic Targets·Peter J NelsonHermann-Josef Gröne
Oct 26, 2012·The Journal of Pathology·Takahisa KawakamiJeremy S Duffield
Jan 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shuyu RenJeremy S Duffield
Jan 18, 2013·American Journal of Physiology. Cell Physiology·Gabriela CampanholleJeremy S Duffield
Mar 29, 2014·Biochemical and Biophysical Research Communications·Paula HernandezFrances M D Henson

❮ Previous
Next ❯

Citations

Apr 10, 2020·Ageing Research Reviews·He-He HuYing-Yong Zhao
Oct 25, 2016·Bone·Aimy Sebastian, Gabriela G Loots

❮ Previous
Next ❯

Related Concepts

Related Feeds