Abstract
To investigate the effect of extracted soybean saponins on the growth of human colon cancer cells. WiDr human colon cancer cells were treated with 150, 300, 600 or 1200 ppm of soy saponin to determine the effect on cell growth, cell morphology, alkaline phosphatase (AP) and protein kinase C (PKC) activities, and P53 protein, c-Fos and c-Jun gene expression. Soy saponin decreased the number of viable cells in a dose-dependent manner and suppressed 12-O-tetradecanol-phorbol-13-acetate-stimulated PKC activity (P < 0.05). Cells treated with saponins developed cytoplasmic vesicles and the cell membrane became rougher and more irregular in a dose-dependent manner, and eventually disassembled. At 600 and 1200 ppm, the activity of AP was increased (P < 0.05). However, the apoptosis markers such as c-Jun and c-Fos were not significantly affected by saponin. Soy saponin may be effective in preventing colon cancer by affecting cell morphology, cell proliferation enzymes, and cell growth.
References
Jan 1, 1990·Anatomy and Embryology·P G Clarke
Nov 1, 1989·Biochemical Pharmacology·Y TanakaP J Higgins
Jan 1, 1994·Journal of Cancer Research and Clinical Oncology·H Y LeeK W Kim
Aug 28, 1997·Oncogene·J PietteV Maréchal
Mar 6, 1998·Oncogene·H J ChaK W Kim
Aug 3, 2002·Nature Reviews. Cancer·Karen H Vousden, Xin Lu
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Eric H Baehrecke
Dec 24, 2002·Phytochemical Analysis : PCA·Mark A BerhowSteven F Vaughn
Dec 24, 2003·Nutrition and Cancer·Hanako KobayashiSharon E Fleming
May 28, 2004·Toxicology and Applied Pharmacology·Shing-Chuan ShenYen-Chou Chen
Oct 9, 2004·Carcinogenesis·Allison A EllingtonKeith W Singletary
Jun 19, 2007·Nutrition and Cancer·Shino ObaShogen Matsushita
May 3, 2008·Gerontology·José Miguel VicencioGuido Kroemer
Dec 17, 2008·The American Journal of Clinical Nutrition·Gong YangWei Zheng
Jan 1, 2009·Nutrition and Cancer·Jayadev Raju, Rekha Mehta
Citations
Mar 19, 2013·Food Chemistry·Yuki KatayanagiShinjiro Imai
Oct 3, 2012·Cell Biochemistry and Function·Gianella CaiozziMarie-Louise Ricketts
Jul 13, 2016·Plant Foods for Human Nutrition·González-Montoya MarcelaMora-Escobedo Rosalva
Nov 14, 2013·ISRN Oncology·Yu-Wei GuoShyh-Hsiang Lin
Oct 18, 2016·Current Opinion in Clinical Nutrition and Metabolic Care·Catherine Bennetau-Pelissero
Apr 23, 2014·Scientific Reports·Keiko TanakaShinjiro Imai
Jul 31, 2014·Journal of Agricultural and Food Chemistry·Cuie GuangShuiyuan Cheng
Nov 11, 2018·The New Phytologist·Jagadeesh SundaramoorthyJong Tae Song
Mar 22, 2012·Journal of Dietary Supplements·Karl KingsleyChristine J Bergman
Jan 18, 2020·International Journal of Molecular Sciences·Samuel Fernández-ToméHang Xiao
Mar 8, 2017·Nutrients·Ana LimaRicardo Boavida Ferreira
Aug 27, 2015·World Journal of Gastroenterology : WJG·Yu-Hua LiQi-Bing Mei
Apr 19, 2018·Journal of Food Science and Technology·Hitoshi HorieShigeo Wada
Jul 1, 2020·Scientific Reports·Leda GuzmánAlejandro Corvalán
Feb 23, 2020·Analytica Chimica Acta·Han-Ju ChienChien-Chen Lai
Nov 30, 2021·Journal of Agricultural and Food Chemistry·Ping S YatesBao-Hua Song