Effect of spacer arm length between adhesion ligand and alginate hydrogel on stem cell differentiation

Carbohydrate Polymers
Jae Won LeeKuen Yong Lee

Abstract

Controlling cell-polymer interactions is one of the most critical components in the development of scaffolds for tissue engineering. In this study, we hypothesized that controlling the spacer arm length of adhesion ligand, such as RGD peptide, coupled to alginate hydrogels could regulate stem cell phenotypes. The results of our investigation indicate that the viability of stem cells was enhanced as spacer arm length increased. The spacer arm length of adhesion ligand also influenced differentiation of stem cells. Osteogenic and adipogenic differentiation of stem cells was promoted by increasing adhesion ligand spacer arm length. On the contrary, chondrogenic differentiation was independent of spacer arm length at the same ligand density and cell concentration. Interestingly, as the cell concentration in alginate gels increased, the gels modified with adhesion ligand with long spacer arms improved chondrogenic differentiation of stem cells. We demonstrate here that the spacer arm length of adhesion ligand conjugated to polymer scaffolds is a key factor in controlling proliferation and differentiation of stem cells, which may be critical for many tissue engineering applications.

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Citations

Nov 21, 2019·Journal of Biomedical Materials Research. Part a·Xintang Zhan
Apr 11, 2020·Tissue Engineering. Part B, Reviews·Jianrui Ma, Chengyang Huang
Jul 23, 2020·Frontiers in Bioengineering and Biotechnology·Mariana Isabel NevesCristina Carvalho Barrias
Jun 10, 2019·Acta Biomaterialia·Kiersten E ScottStephanie I Fraley
Jul 9, 2021·Acta Biomaterialia·Xingzhen Zhang, Sabine van Rijt

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