PMID: 8581986Oct 1, 1995Paper

Effect of specific binding of high density lipoprotein to eel hepatocytes on their secretion of lipoprotein

Cell Structure and Function
N JibrilS Hayashi

Abstract

Specific binding of eel serum high-density lipoprotein (HDL) to eel hepatocytes was demonstrated by using a synthesized fluorescent lipophilic dye. HDL binding was inhibited by the addition of unlabeled HDL. The binding of HDL to the hepatocytes was saturated at concentrations over 100 micrograms HDL protein/ml and Kd value was 20 micrograms HDL protein/ml. A fluorescent photomicrograph of the cultured eel hepatocytes which were incubated with the dye showed the bright, circumferential plasma membranes stained with the dye. 125I-HDL was incorporated into the acid insoluble- and soluble-fractions of the cultured hepatocytes during incubation at 28 degrees C for 1 h. There are three remarkable characteristics of the effect of HDL on the cultured hepatocytes. One is that the addition of HDL to the hepatocytes induced the efflux of cholesterol, triacylglycerol, and phospholipid from the hepatocytes. The second characteristic is that the efflux of the intracellular lipids was carried out with very-low-density-like or chylomicron-like lipoprotein secreted by the hepatocytes. The third characteristic is that HDL specifically stimulated the synthesis of the lipoprotein and had no effect on the synthesis of intracellular proteins and th...Continue Reading

Citations

May 15, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Akiko KumagaiSeiichi Hayashi
Mar 15, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Akiko Kumagai, Seiichi Hayashi
Feb 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·D Ndiaye, S Hayashi
Mar 24, 2009·IEEE Transactions on Bio-medical Engineering·Nikita S Imennov, Jay T Rubinstein
Jul 25, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·D NdiayeS Hayashi
Jul 4, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·H KatohS Hayashi
Aug 28, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jyh-Yih ChenJen-Leih Wu

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