Effect of Stabilization on the Healing Process of Femur Fractures in Aged Mice

Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research
Tina HistingMichael D Menger

Abstract

The influence of mechanical stability on fracture healing has previously been studied in adult mice, but is poorly understood in aged animals. Therefore, we herein studied the effect of stabilization on the healing process of femur fractures in aged mice. Twenty-four 18-month-old CD-1 mice were stabilized after midshaft fracture of the femur with an intramedullary screw. In another 24 18-month-old mice, the femur fractures were left unstabilized. Bone healing was studied by radiological, biomechanical, histomorphometric, and protein expression analyses. After 2 and 5 weeks of healing, the callus of nonstabilized fractures compared to stabilized fractures was significantly larger, containing a significantly smaller amount of osseous tissue and a higher amount of cartilaginous tissue. This was associated with a significantly lower biomechanical stiffness during the early phase of healing. However, during the late phase of fracture healing both nonstabilized and stabilized fractures showed a biomechanical stiffness of ∼40%. Of interest, Western blot analyses of callus tissue demonstrated that the expression of proteins related to angiogenesis, bone formation and remodeling, i.e. VEGF, CYR61, BMP-2, BMP-4, Col-2, Col-10, RANKL, OPG...Continue Reading

References

Jan 26, 1999·Clinical Orthopaedics and Related Research·D M Nunamaker
Jan 26, 1999·Clinical Orthopaedics and Related Research·L E ClaesP Augat
Jan 12, 2001·International Orthopaedics·G Mora, F Forriol
Jun 12, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R A MeyerG M Kiebzak
Oct 18, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Lutz ClaesPeter Augat
Jul 10, 2003·The Journal of Bone and Joint Surgery. American Volume·Ralph A MeyerPatricia Garges
Oct 14, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Peter AugatLutz Claes
May 10, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H SchellG N Duda
May 12, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jasmin LienauHermann J Bail
Jun 7, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Chuanyong LuRalph S Marcucio
Sep 15, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Louis C GerstenfeldThomas A Einhorn
Aug 9, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ralph A MeyerMartha H Meyer
Nov 10, 2006·Experimental Gerontology·Reinhard GruberJeffrey O Hollinger
Sep 18, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Matthias SchoenGeorg Gradl
May 9, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Chuanyong LuRalph S Marcucio
Jul 29, 2008·The Journal of Surgical Research·Joerg H HolsteinMichael D Menger
Oct 14, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Amish A NaikRegis J O'Keefe
Mar 11, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jasmin LienauHanna Schell
Sep 26, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Tina HistingMichael D Menger
Jul 9, 2011·Journal of Orthopaedic Trauma·Chuanyong LuRalph S Marcucio
Apr 25, 2012·Calcified Tissue International·T HistingM D Menger
Jan 1, 2013·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Tina HistingMichael D Menger
Aug 12, 2014·Clinical Orthopaedics and Related Research·Luke A LopasJaimo Ahn
Mar 4, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Moritz KleinTina Histing

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Citations

May 24, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Edoardo BorgianiSara Checa
Jul 15, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·John H HebbJaimo Ahn
Dec 7, 2018·Hand : Official Journal of the American Association for Hand Surgery·Ronit WollsteinDuffield Ashmead
Dec 3, 2016·Innovative Surgical Sciences·Melanie Haffner-LuntzerAnita Ignatius

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