Effect of starvation on the endocytic pathway in Dictyostelium cells.

Eukaryotic Cell
Ewan W SmithPierre Cosson

Abstract

Dictyostelium discoideum amoebae have been used extensively to study the structure and dynamics of the endocytic pathway. Here, we show that while the general structure of the endocytic pathway is maintained in starved cells, its dynamics rapidly slow down. In addition, analysis of apm3 and lvsB mutants reveals that the functional organization of the endocytic pathway is profoundly modified upon starvation. Indeed, in these mutant cells, some of the defects observed in rich medium persist in starved cells, notably an abnormally slow transfer of endocytosed material between endocytic compartments. Other parameters, such as endocytosis of the fluid phase or the rate of fusion of postlysosomes to the cell surface, vary dramatically upon starvation. Studying the endocytic pathway in starved cells can provide a different perspective, allowing the primary (invariant) defects resulting from specific mutations to be distinguished from their secondary (conditional) consequences.

References

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Feb 21, 2002·Molecular Biology of the Cell·Edward HarrisJames Cardelli
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Sep 14, 2006·Journal of Cell Science·Valentina MercantiPierre Cosson
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Citations

Jan 25, 2012·BMC Developmental Biology·Pundrik JaiswalRamamurthy Baskar
Apr 30, 2010·BMC Research Notes·Valentina Mercanti, Pierre Cosson
Aug 14, 2013·BMC Research Notes·Alexandre Vernay, Pierre Cosson
Jan 30, 2018·Frontiers in Cellular and Infection Microbiology·Elena Cardenal-MuñozThierry Soldati
Jan 23, 2018·Frontiers in Immunology·Joe Dan DunnThierry Soldati
May 23, 2015·Journal of Cell Science·Jackie PerrinPierre Cosson

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