Effect of Static Compression Loads on Intervertebral Disc: An in Vivo Bent Rat Tail Model

Orthopaedic Surgery
Wei XiaHui-Lin Yang

Abstract

To evaluate how well different magnitudes of compression-induced degenerative changes using a bent rat tail model simulated human lumbar lordosis. It has been shown that compression plays an important role in intervertebral disc degeneration (IDD). Sprague-Dawley rats (n = 25) were instrumented with a special compressive apparatus that was used to bend the intervertebral disc between the 8th and the 10th caudal vertebral bodies using two Kirschner wires inserted percutaneously into the middle of two tail vertebrae. Then, rats were divided into five different static compression loads (control, sham, 1.8 N, 4.5 N, and 7.2 N). The degeneration of the discs was evaluated by magnetic resonance imaging (MRI), histology, gene expression of anabolism and catabolism after 2 weeks. We used the signal characteristics of the disc in T2-weighted MRI to reflect the changes caused by degeneration as this is the most relevant and clinically recognized way to assess IDD. Pfirrmann classification was used to classify disc images. The tail discs from C8-9 and C9-10 with their two adjacent half vertebrae were carefully cut out and decalcified. Then the sections were paraffin-embedded and cut into 5-μm sections by histotome. Finally, they were stai...Continue Reading

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Citations

Oct 30, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ting LiangZong-Ping Luo
May 30, 2019·BioMed Research International·Xiaoyang TongJun Zou
Jul 27, 2021·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Yidian WangXuewen Kang

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