Effect of stereotactic radiosurgery on lipids and proteins of normal and hypoperfused rat brain homogenates: a Fourier transform infrared spectroscopy study

International Journal of Radiation Biology
Neslihan ToyranFeride Severcan

Abstract

The effect of stereotactic radiosurgery on lipids and proteins of normal and hypoperfused rat brain was investigated to see if hypoxic areas are really more resistant to radiation effects or not. Rat brain samples from control, stereotactically irradiated and chronically hypoperfused plus stereotactically irradiated groups were homogenized separately with saline phosphate buffer, and centrifuged at 125,000 g for 15 min. Membrane rich parts (pellet) of these homogenates were used for Fourier Transform Infrared (FTIR) spectroscopy studies. Mann-Whitney U tests were performed on the groups, two by two, to test the significance of the differences between the control group and stereotactically irradiated group as well as the control group and chronically hypoperfused plus stereotactically irradiated group. After a single high dose of X-rays to healthy rat brain, the lipid concentration increased slightly, protein content decreased significantly (p < 0.05) and protein-to-lipid ratio decreased slightly. The secondary structure of the proteins was altered in the irradiated brain samples such that the content of a-helical structure decreased significantly (p < 0.01) and random coil increased dramatically (p < 0.05). The effect of radiat...Continue Reading

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Citations

Feb 26, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yusuf BaranAylin Camgoz
Sep 8, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·N KrishnakumarM G Arun Manohar
Dec 4, 2015·European Biophysics Journal : EBJ·Tahani H DakhakhniSafaa Y Qusti
Jul 15, 2015·Environmental Science & Technology·Yuming ChenQixing Zhou
Jun 21, 2012·Technology in Cancer Research & Treatment·Cagatay CeylanYusuf Baran
Jun 8, 2014·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Jean Régis
Jun 1, 2010·Journal of Neuro-oncology·Jean RégisMichael Park
Jan 24, 2007·Archives of Biochemistry and Biophysics·Neslihan ToyranFeride Severcan
Apr 28, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P L R M Palaniappan, V Vijayasundaram
Jun 24, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P L R M Palaniappan, K S Pramod
Mar 23, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Pl Rm Palaniappan, K S Pramod

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