PMID: 8972708Dec 4, 1996Paper

Effect of sterical stabilization on macrophage uptake in vitro and on thickness of the fixed aqueous layer of liposomes made from alkylphosphocholines

Biochimica Et Biophysica Acta
R ZeisigD Arndt

Abstract

A serious problem using liposomes for therapeutic purposes is the fast removal from blood circulation by components of the reticuloendothelial system (RES) most likely after opsonization of the vesicles. This study was performed to quantify the reduction in macrophage uptake in vitro of sterically stabilized liposomes (PEG-liposomes) prepared from hexadecylphosphocholine, cholesterol and poly(ethylene glycol2000) distearoylphosphoethanolamine (PEG2000DSPE) for the first time. The uptake was determined using HPC-liposomes of different defined size (125, 250 and 1000 nm) without and with sterical stabilization by incorporating 5 mol% of PEG2000DSPE. HPTS was used as fluorescence marker allowing the discrimination between general uptake and the part of liposomes internalized into the low pH-compartment (Daleke, L.D., Hong, K. and Papahadjopoulos. D. (1990) Biochim. Biophys. Acta 1024, 352-366). Liposomal uptake by J774 mouse macrophage-like cells was time-dependent. Both the uptake and internalization were clearly reduced for PEG-liposomes compared to plain liposomes. Sterical stabilization reduced the general uptake of liposomes in vitro by more than 50% and the internalization by about 50-60%. PEG-liposomes additionally showed a...Continue Reading

References

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Citations

Feb 26, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Neila M Silva-BarcellosFrédéric Frézard
Aug 23, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yasuyuki SadzukaTakashi Sonobe
Dec 18, 2001·Advanced Drug Delivery Reviews·K Kono
Jun 14, 2002·International Journal of Pharmaceutics·Tatiana S LevchenkoVladimir P Torchilin
Feb 26, 2004·Drug Discovery Today·Fabrizio Marcucci, François Lefoulon
Jul 9, 2008·Journal of Microencapsulation·Maha NasrA A Elshamy
Jan 29, 2013·Journal of Liposome Research·Arash MomeniAlireza Khoshdel
Jul 6, 2011·Drug Development and Industrial Pharmacy·Sohail AkhterFarhan J Ahmad
Oct 11, 2011·Advanced Drug Delivery Reviews·Sandrine DufortJean-Luc Coll
Jan 27, 2010·International Journal of Pharmaceutics·Yousuke OmokawaKeiichi Kato
May 20, 2008·International Journal of Pharmaceutics·Tamer ShehataToshikiro Kimura
Mar 6, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Keiji HirotaHiroshi Terada
Apr 28, 2004·Advanced Drug Delivery Reviews·Evan C UngerReena Zutshi
Feb 7, 2006·International Journal of Pharmaceutics·Yasuyuki SadzukaTakashi Sonobe
Dec 1, 2012·International Journal of Pharmaceutics·Ikumi Sugiyama, Yasuyuki Sadzuka
Jul 25, 2014·Journal of Liposome Research·Ghada Ahmed Abdelbary, Mona Hassan Aburahma
Oct 1, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·David R WilsonRebecca A Bader
Sep 17, 2013·International Journal of Pharmaceutics·Matthias Wacker
Mar 23, 2015·International Journal of Pharmaceutics·Abdulaziz M Al-MahallawiMona H Aburahma
Jan 7, 2005·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yasuyuki SadzukaTakashi Sonobe
Jan 5, 2007·Biological & Pharmaceutical Bulletin·Ikumi Sugiyama, Yasuyuki Sadzuka
Feb 28, 2002·The Journal of Pharmacology and Experimental Therapeutics·Wai Ming LiMarcel B Bally
Nov 9, 2017·Drug Development and Industrial Pharmacy·Azza Abdelmageed MatloubHanaa Hamdy Ahmed

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