Effect of storage and ultraviolet B irradiation on CD14-bearing antigen-presenting cells (monocytes) in platelet concentrates

Transfusion
E Fiebig, T A Lane

Abstract

Ultraviolet B (UVB) irradiation of platelet concentrate (PCs) reduces platelet alloimmunization, but the mechanism of the effect is unclear. Evidence suggests that UVB may downregulate the expression of surface adhesion molecules on passenger antigen-presenting cells in PCs. The effect of blood bank storage, platelet preparation from whole blood, and UVB irradiation on the quantitative expression of intercellular adhesion molecule-1 (ICAM-1, or CD54), HLA-DR, CD45, and CD11c on CD14-positive antigen-presenting cells (monocytes) was studied by using two-color flow cytometry. Blood bank storage for 4 days resulted in upregulation of ICAM-1 and HLA-DR and downregulation of CD14 but left the expression of CD11c and CD45 unchanged. Preparation of PCs from fresh whole blood was associated with a rapid increase in CD11c without upregulation of ICAM-1 and HLA-DR. UVB irradiation before storage inhibited the upregulation of ICAM-1 and HLA-DR, resulted in accelerated downregulation of CD14, and was associated with increased loss of monocytes. Agitation of the PC bag during irradiation was of critical importance, since omission of agitation resulted in largely uninhibited upregulation of ICAM-1 but was still associated with significantly ...Continue Reading

References

Feb 1, 1991·European Journal of Immunology·M HertlC A Elmets
May 1, 1990·The Journal of Clinical Investigation·J KrutmannC A Elmets
Aug 2, 1990·Nature·T A Springer
Jul 1, 1985·Transfusion·A B Champion, R A Carmen
Mar 1, 1981·The Journal of Investigative Dermatology·W AbererK Wolff
Jan 1, 1994·Transfusion·G Stack, E L Snyder

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Citations

Jan 10, 2003·Transfusion·Hiromichi ArigaUNKNOWN Viral Activation Transfusion Study
May 1, 2004·Journal of Infusion Nursing : the Official Publication of the Infusion Nurses Society·Girolamo A OrtolanoBarry Wenz
Oct 8, 2020·Clinical Reviews in Allergy & Immunology·Alberto BorettiStefania Castelletto

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