PMID: 6412408Jun 1, 1983Paper

Effect of storage of sputum specimens at room temperature on smear and culture results

Tubercle
C N ParamasivanS P Tripathy

Abstract

A study of 41 specimens of sputum stored for up to 28 days at room temperature in a tropical country showed that smear results were not affected by storage, the positivity being 83% before storage and 80-83% after. The culture positivity was 88% before storage, and 83%, 68%, 22%, 13% and 0% after 3, 7, 14, 21 and 28 days of storage respectively. The reduction in positivity on storage attains significance at 7 days (P = 0.05). Another study involved 163 specimens of sputum and storage for 3, 5 or 7 days. Each specimen was examined before storage and after 2 periods of storage, at random. The smear results were again not affected. There was, however, significant loss of viability, the proportion culture positive being reduced from 92% before storage to 83% at 3 days (P = 0.05), 71% at 5 days (P less than 0.01) and 63% at 7 days (P less than 0.001). The contamination rate was 5%, 7%, 12% and 18% respectively. It is concluded that sputum should not be stored at room temperature for longer than 3 days for culture but it can be stored for 4 weeks without any loss of smear-positivity.

Citations

Nov 19, 2015·Frontiers in Microbiology·Shady Asmar, Michel Drancourt
Sep 24, 2015·Acta Tropica·Norbert Georg SchwarzHagen Frickmann
Apr 21, 2007·Tropical Medicine & International Health : TM & IH·Marieke J van der Werf, Martien W Borgdorff
Jan 1, 2014·International Journal of Bacteriology·Gomathi SekarN Selvakumar
Feb 14, 2015·Clinics in Chest Medicine·Jakko van Ingen
May 1, 2016·Journal of Epidemiology and Global Health·Bhagwan MaharjanCassandra D Kelly-Cirino
Aug 9, 2017·African Journal of Laboratory Medicine·Ishani PathmanathanHeather Alexander

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