Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood

Prostaglandins, Leukotrienes, and Essential Fatty Acids
M NurhasanL Lauritzen

Abstract

Fatty acid analysis requires standardized collection and storage of samples, which can be a challenge under field conditions. This study describes the effect of storage temperature on fatty acid composition in two sets of whole blood samples collected from 66 children in a rural area in Cambodia. The samples were stored with butylated hydroxytoluene at -20 °C and -80 °C and the latter required extra transfers due to storage facility limitation. Fatty acid composition was analyzed by high-throughput gas-chromatography and evaluated by paired t-tests and Bland-Altman plots. Total amounts of fat in -20 °C and -80 °C samples did not differ, but there was relatively more highly unsaturated fatty acids (15.8 ± 2.7 vs. 14.4 ± 2.5%, p < 0.001) and a lower n-6/n-3 ratio (6.4 ± 1.4 vs. 6.9 ± 1.4, p < 0.001) in the -20 °C samples. Our results indicate that the importance of storage temperature should be evaluated in the context of storage facility availability and risk of temperature fluctuations during transport.

References

Sep 12, 2009·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Adam H MetherelKen D Stark
Jul 20, 2010·Lancet·Palaniappan SaravananPhilip C Calder
May 25, 2012·The British Journal of Nutrition·Javier Delgado-ListaFrancisco Perez-Jimenez
Sep 13, 2012·JAMA : the Journal of the American Medical Association·Evangelos C RizosMoses S Elisaf
Sep 21, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·H L HarbildL Lauritzen

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Citations

Jan 24, 2016·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Adam H Metherel, Ken D Stark
Jan 24, 2016·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Ken D StarkLouise Pilote

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