Effect of strain on actomyosin kinetics in isometric muscle fibers

Biophysical Journal
Verl SiththanandanM A Ferenczi

Abstract

Investigations were conducted into the biochemical and mechanical states of cross-bridges during isometric muscle contraction. Rapid length steps (3 or 6 nm hs(-1)) were applied to rabbit psoas fibers, permeabilized and isometric, at either 12 degrees C or 20 degrees C. Fibers were activated by photolysis of P(3)-1-(2-nitrophenyl)-ethyl ester of ATP infused into rigor fibers at saturating Ca(2+). Sarcomere length, tension, and phosphate release were recorded-the latter using the MDCC-PBP fluorescent probe. A reduction in strain, induced by a rapid release step, produced a short-lived acceleration of phosphate release. Rates of the phosphate transient and that of phases 3 and 4 of tension recovery were unaffected by step size but were elevated at higher temperatures. In contrast the amplitude of the phosphate transient was smaller at 20 degrees C than 12 degrees C. The presence of 0.5 or 1.0 mM added ADP during a release step reduced both the rate of tension recovery and the poststep isometric tension. A kinetic scheme is presented to simulate the observed data and to precisely determine the rate constants for the elementary steps of the ATPase cycle.

References

Jan 1, 1986·Annual Review of Biophysics and Biophysical Chemistry·M G Hibberd, D R Trentham
Nov 1, 1985·Biophysical Journal·R Cooke, E Pate
Oct 22, 1971·Nature·A F Huxley, R M Simmons
Oct 21, 2003·The Journal of Physiology·Timothy G WestRoger C Woledge
Nov 25, 2004·Progress in Biophysics and Molecular Biology·Nicholas P SmithDenis S Loiselle
Dec 15, 2004·Biophysical Journal·Andrey K TsaturyanSergey Y Bershitsky
Jan 12, 2005·Structure·Michael A FerencziAndrey K Tsaturyan

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Citations

May 29, 2008·Annals of Biomedical Engineering·D A Smith, S M Mijailovich
Dec 29, 2011·Progress in Biophysics and Molecular Biology·G F Elliott, C R Worthington
Jun 23, 2015·The Journal of Physiology·Vasco SequeiraJolanda van der Velden
Jun 10, 2008·Biophysical Journal·Natalia A KoubassovaAndrey K Tsaturyan
Nov 22, 2011·Biophysical Journal·Dale C BickhamMichael A Ferenczi
Mar 29, 2008·The Journal of Physiology·Alison K StubbingsMichael A Ferenczi
Sep 18, 2017·Journal of Muscle Research and Cell Motility·Robert Stehle, Chiara Tesi
Jan 24, 2008·The Journal of Biological Chemistry·Máté GyimesiAndrás Málnási-Csizmadia

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