Effect of stretching on gene expression of beta1 integrin and focal adhesion kinase and on chondrogenesis through cell-extracellular matrix interactions

European Journal of Cell Biology
I TakahashiH Mitani

Abstract

Differentiation of skeletal tissues, such as bone, ligament and cartilage, is regulated by complex interaction between genetic and epigenetic factors. In the present study, we attempted to elucidate the possible role of cell-extracellular matrix (ECM) adhesion on the inhibitory regulation in chondrogenesis responding to the tension force. The midpalatal suture cartilages in rats were expanded by orthopedic force. In situ hybridization for type I and II collagens, immunohistochemical analysis for fibronectin, alpha5 and beta1 integrins, paxillin, and vinculin, and cytochemical staining for actin were used to demonstrate the phenotypic change of chondrocytes. Immunohistochemical analysis for phosphorylation and nuclear translocation of extracellular signal-regulated kinase (ERK)-1/2 was performed. The role of the cell-ECM adhesion in the response of the chondroprogenitor cells to mechanical stress and the regulation of gene expression of focal adhesion kinase (FAK) and integrins were analyzed by using an in vitro system. A fibrous suture tissue replaced the midpalatal suture cartilage by the expansive force application for 14 days. The active osteoblasts that line the surface of bone matrix in the newly formed suture tissue stron...Continue Reading

References

Dec 1, 1986·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·T Asano
Nov 1, 1982·Developmental Biology·M SolurshK L Jensen
May 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J Wroblewski, C Edwall-Arvidsson
Dec 1, 1994·Experimental Cell Research·S TavellaP Castagnola
Sep 1, 1994·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·K IgarashiH Shinoda
Jul 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·A J BanesL Miller
Dec 1, 1995·Matrix Biology : Journal of the International Society for Matrix Biology·M Pacifici
Oct 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T YasudaT Nakamura
Feb 1, 1997·Current Opinion in Cell Biology·K M Yamada, B Geiger
Dec 6, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M S HirschK K Svoboda
Aug 1, 1997·The International Journal of Biochemistry & Cell Biology·J L Guan
Nov 18, 1998·Current Opinion in Cell Biology·C G Galbraith, M P Sheetz
Dec 29, 1998·The Journal of Cell Biology·J H ZhaoJ L Guan
Apr 15, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·I SembaH C Slavkin
Aug 10, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H S LeeD M Salter
Sep 16, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E LangelierM D Buschmann
Mar 30, 2001·The Journal of Biological Chemistry·M ShakibaeiH J Merker
Nov 21, 2001·Nature Reviews. Molecular Cell Biology·B GeigerK M Yamada
Jan 10, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M S KurtisR L Sah
Mar 19, 2002·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Emi ImotoMasao Nagumo

❮ Previous
Next ❯

Citations

May 12, 2007·Journal of Cellular Physiology·Anita WoodsFrank Beier
Jan 26, 2008·Journal of Dental Research·M Rubia MarquesM Fagundes Santos
May 14, 2009·Journal of Biomedical Materials Research. Part a·Cheng Zhe JinByoung-Hyun Min
Jun 22, 2010·Cell Communication & Adhesion·Kelly C ClauseKimimasa Tobita
Oct 20, 2011·Orthodontics & Craniofacial Research·W-J ZhongL Wang
Nov 4, 2011·The Journal of Biological Chemistry·Ichiro NishimuraHidetoshi Okabe
Dec 17, 2008·Connective Tissue Research·Maosheng Xia, Yue Zhu
Sep 20, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Juan Sanchez-EstebanDonald E Ingber
Apr 14, 2006·Cells, Tissues, Organs·Lynne A Opperman, Joseph T Rawlins
Apr 26, 2015·Molecular and Cellular Biochemistry·Huajiang ChenWen Yuan
Dec 5, 2018·Stem Cells International·Chang LiuYu-Long Sun
Mar 28, 2006·The Journal of Biological Chemistry·Anita Woods, Frank Beier
Oct 31, 2019·Tissue Engineering. Part B, Reviews·Aleena A Saidova, Ivan A Vorobjev
Mar 3, 2007·Gene·James H-C WangHee-Jeong Im
Oct 12, 2018·Science Translational Medicine·Eun Kyung SongTae Joo Park
May 24, 2011·Developmental Biology·R Christian SolemRichard A Schneider

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.