Effect of structure of the initiator codon on translation in E. coli

FEBS Letters
Khudyakov YuEV D Smirnov


A set of plasmids carrying different initiator codons--either AUG, or GUG, or UUG, or CUG (as a control) in the hybrid gene lacIZ--was constructed by using synthetic oligonucleotides. GUG and UUG codons were demonstrated to be 2-3 times less effective than AUG in translation initiation. Furthermore, the correlation between the efficiencies of different initiator codons in translation initiation proved to vary, depending on the phase of bacterial growth. The rarely occurring usage in nature of the initiator codons GUG and UUG is supposed to be due to the particular role played by the initiator triplets in regulation of gene expression.


May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A La TeanaClaudio O Gualerzi
Nov 3, 2015·Journal of Molecular Biology·Michelle A Kriner, Eduardo A Groisman
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Jun 3, 2017·Nucleic Acids Research·He ChengguangClaudio O Gualerzi


Mar 3, 1986·FEBS Letters·A C Looman, P H van Knippenberg
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Related Concepts

RNA Conformation
Alkalescens-Dispar Group
Protein Biosynthesis
Codon, Initiator
Sense Codon
Codon (Nucleotide Sequence)
DNA Conformation

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