Effect of structure of the initiator codon on translation in E. coli

FEBS Letters
Khudyakov YuEV D Smirnov

Abstract

A set of plasmids carrying different initiator codons--either AUG, or GUG, or UUG, or CUG (as a control) in the hybrid gene lacIZ--was constructed by using synthetic oligonucleotides. GUG and UUG codons were demonstrated to be 2-3 times less effective than AUG in translation initiation. Furthermore, the correlation between the efficiencies of different initiator codons in translation initiation proved to vary, depending on the phase of bacterial growth. The rarely occurring usage in nature of the initiator codons GUG and UUG is supposed to be due to the particular role played by the initiator triplets in regulation of gene expression.

References

May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A La TeanaClaudio O Gualerzi
Nov 3, 2015·Journal of Molecular Biology·Michelle A Kriner, Eduardo A Groisman
Jun 26, 1990·Biochemistry·C O Gualerzi, C L Pon
Jun 3, 2017·Nucleic Acids Research·He ChengguangClaudio O Gualerzi

Citations

Mar 3, 1986·FEBS Letters·A C Looman, P H van Knippenberg
Jul 1, 1985·European Journal of Biochemistry·G WeyensA Piérard
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P ReddyK McKenney
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L GoldR Saunders

Related Concepts

RNA Conformation
Alkalescens-Dispar Group
Protein Biosynthesis
Codon, Initiator
Sense Codon
Codon (Nucleotide Sequence)
Oligonucleotides
DNA Conformation
Plasmids
Cistron

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