Effect of sub-anesthetic doses of isoflurane and propofol on postoperative cognition and underlying mechanism in rats with mild cognitive impairment

Zhonghua yi xue za zhi
S Y LiuG L Wang

Abstract

To study the effect of sub-anesthetic doses of isoflurane and propofol on the postoperative cognitive function of rats with mild cognitive impairment (MCI) following orthopedic operation. Male sprague-dawley rats (n=100) were randomly divided into sham group and model group.Bilateral carotid artery was separated with no bilateral narrowing in the sham operation group while bilateral carotid artery was occluded in model group.After 30 days, Morris Water Maze test were used to select MCI rats. The proportion of the value that average escape latency in model group minused average escape latency in sham group less than 20% was marked as MCI rats. After 36 days, the rats with mild cognitive impairment were randomly divided into 4 groups (n=18). Iso group: Isoflurane (1.9%) 3 h, combination group: propofol (20 mg·kg(-1)·h(-1))+ isoflurane (1%) 3 h. Inhibitor with these two groups were respectively used the selective KCC2 inhibitor-DIOA 30 μg 15 min before anesthesia. Then rats received an open tibial fracture on the hind paw with an intramedullary fixation. The rats in sham group were only sutured skin incision. At the day 7 postoperatively, the Y maze test were used to test the change of cognition, nissl staining was used to observe...Continue Reading

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