Effect of subcritical water extraction conditions on the activity of alcohol metabolizing enzymes, ACE inhibition, and tyrosinase inhibition in Protaetia brevitarsis larvae.

Food Science and Biotechnology
Sung-Mun Bae, Seung-Cheol Lee

Abstract

In order to develop processing methods with high physiological activity for Protaetia brevitarsis larvae (PBL), subcritical water (SCW) extraction was applied. The dried powder (1 g) of PBL was extracted with 10 mL distilled water at 100, 200, and 300 °C for 30 min. The SCW treatment significantly (p < 0.05) increased some physiological activities of the PBL extracts. The SCW extract at 300 °C increased alcohol dehydrogenase, acetaldehyde dehydrogenase, and tyrosinase inhibitory activities from 192.3 ± 4.1% to 452.2 ± 0.5%, 125.4 ± 2.9% to 153.3 ± 0.4%, and - 7.0 ± 0.7% to 26.1 ± 1.4%, respectively, compared to the extract at 100 °C. Contrarily, the inhibition activity of angiotensin converting enzyme was the highest at 200 °C. These results suggest that SCW is a suitable method to extract and maintain the physiological activity of PBL.

References

Jul 27, 1975·Biochimica Et Biophysica Acta·T Koivula, M Koivusalo
Aug 1, 1991·Alcoholism, Clinical and Experimental Research·C S Lieber
Jul 1, 1971·Biochemical Pharmacology·D W Cushman, H S Cheung
Mar 21, 2017·Food Chemistry·Seung Yun Lee, Sun Jun Hur

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