Effect of substrate and cell surface hydrophobicity on phosphate utilization in bacteria.

Applied and Environmental Microbiology
M J LemkeR G Wetzel

Abstract

We measured the rates of utilization of hydrophobic and hydrophilic phosphate compounds in gram-negative bacteria with different surface hydrophobicities, isolated from wetland habitats. Three hydrophobic and two hydrophilic bacterial species were selected for study by measuring cell adherence to hydrocarbons. The bacteria were grown under phosphorus-limited conditions with P(infi), hydrophilic (beta)-glycerophosphate, or hydrophobic phosphatidic acid as the phosphate source. Hydrophilic bacteria grew most rapidly on P(infi), followed by (beta)-glycerophosphate. Phosphatidic acid did not support growth or did so at a much later time (40 h) than did the other phosphate treatments. Although all hydrophobic species grew well on these substrates, the rate of growth of two Acinetobacter baumannii isolates on phosphatidic acid exceeded the rate of growth on phosphate or (beta)-glycerophosphate. A membrane phospholipid and lipopolysaccharide were used as a source of phosphorus by hydrophobic species, whereas hydrophilic species could not use the membrane phospholipids and used lipopolysaccharide to a lesser extent. Besides hydrophobic interaction between cells and substrate, phosphatase activity, which was cell bound in hydrophilic sp...Continue Reading

References

Mar 1, 1985·Microbiological Reviews·H Nikaido, M Vaara
Jun 1, 1994·Applied and Environmental Microbiology·Y Zhang, R M Miller
Apr 1, 1994·Applied and Environmental Microbiology·M J MeadeJ P Nakas

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Citations

Aug 22, 2001·Brazilian Journal of Biology = Revista Brasleira De Biologia·S M ThomazT A Pagioro
Dec 18, 2019·Environmental Science and Pollution Research International·Didier TécherMarie Le Jean

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