Effect of substrate exposure and other growth condition manipulations on norA expression

The Journal of Antimicrobial Chemotherapy
G W Kaatz, Susan M Seo

Abstract

Multidrug efflux is a resistance mechanism that simultaneously affects susceptibility to many structurally unrelated compounds. The regulation of norA expression, which encodes the Staphylococcus aureus NorA multidrug efflux pump, is not well understood but the MgrA global regulator and the arlRS locus are involved. The expression of genes encoding proteins related to NorA, such as QacA of S. aureus and Bmr of Bacillus subtilis, is affected by pump substrates. In these instances, substrate interacts with regulatory proteins such that pump gene transcription is increased. The goal of this study was to identify if a similar substrate-level effect exists, or an effect of other growth condition manipulations, on the expression of norA. A transcriptional fusion between norA and lacZ was created in single copy on the chromosome of S. aureus SH1000. beta-Galactosidase activity was quantified following exposure of the fusion strain to various NorA substrates, salicylate, a high salt concentration, putative soluble factors elaborated during growth, and different incubation temperatures. Exposure to several substrates significantly increased norA expression whereas salicylate and osmotic stress had no effect and no stable soluble factor ...Continue Reading

Citations

Dec 22, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Dana CervinkovaZoran Jaglic
Oct 24, 2006·Journal of Bacteriology·James T RiordanJohn E Gustafson
Jun 26, 2007·Journal of Molecular Microbiology and Biotechnology·Karl A HassanMelissa H Brown
Aug 15, 2009·Drugs·Xian-Zhi Li, Hiroshi Nikaido
Mar 20, 2012·Microbial Pathogenesis·Marco A Juárez-VerdayesJuan C Cancino-Diaz
Jun 17, 2006·International Journal of Antimicrobial Agents·James T RiordanJohn E Gustafson
Jan 9, 2008·FEMS Microbiology Letters·Dacheng WangXuming Deng
Jul 28, 2016·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Bryan D Schindler, Glenn W Kaatz
Apr 10, 2013·The Open Microbiology Journal·Sofia Santos CostaIsabel Couto
Jun 24, 2021·Journal of Bioenergetics and Biomembranes·Cristina Rodrigues Dos Santos BarbosaFrancisco Assis Bezerra da Cunha

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