Effect of sucrose on formation of the beta-amyloid fibrils and D-aspartic acids in Abeta 1-42

Biological & Pharmaceutical Bulletin
Tadashi UedaTaiji Imoto

Abstract

Beta-amyloid peptide 1-42 is a major peptide constituent of beta-amyloid fibrils. We investigated the role of sucrose on the deposition and the D-aspartic acid formation in an amyloidogenic peptide 1-42 under physiological conditions. From analyses using thioflavine-T fluorometric assay and electronmicroscopic spectroscopy after 60 h incubation at 37 degrees C, it was found that sucrose retarded the fibril formation in the amyloidogenic peptide. The retardation of the formation of amyloid fibrils by sucrose was suggested to be not due to viscosity but due to disturbance of the assemlby of alpha-helix containing peptides. Moreover, we showed that the formation of D-aspartyl residue, which is found in beta-amyloid fibrils from Alzheimer disease brains, in the amyloidogenic peptide was also retarded in the presence of sucrose.

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Citations

Dec 12, 2002·Micron : the International Research and Review Journal for Microscopy·J R Harris
Jan 26, 2016·Journal of Agricultural and Food Chemistry·Jihua LiuJohn A Carver
Feb 8, 2013·Protein Science : a Publication of the Protein Society·Masahiro AbeTadashi Ueda
Sep 23, 2020·International Journal of Biological Macromolecules·Chien-Yu LinSteven S-S Wang
Aug 21, 2013·International Journal of Biological Macromolecules·Nitin K PandeySwagata Dasgupta
Aug 18, 2021·The International Journal of Neuroscience·Rimaljot SinghTanzeer Kaur
Dec 8, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jasjeet Kaur SahniCharles Ramassamy

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