PMID: 6412976Jan 1, 1983Paper

Effect of supplementation of culture media with polyunsaturated fatty acids on the morphology and composition of cell cultures of fetal mouse brain

Comptes rendus des séances de l'Académie des sciences. Série III, Sciences de la vie
J M BourreA Tixier-Vidal

Abstract

Dissociated cell cultures were grown from fetal Mouse Cerebral hemisphere taken on the 16th day of gestation. Cells grown in chemically defined medium (MCD) for 8 days contain half the polyunsaturated fatty acids (PUFA) (in %) found at day 0. Cells contain n-9 trienes demonstrating that they are grown under conditions of essential fatty acid deficiency. The reduced amount of PUFA in MCD cells is balanced by an increase in monounsaturated fatty acids. The satured fatty acids are not affected. The PUFA present in the starting cells are partly preserved and reutilized (70%). Adding fatty acids of the n-6 series increases the content of n-6 fatty acids in the cells, but also provokes an increase in the n-3 fatty acids. Among several combinations of fatty acids, only the mixture of 20:4 and 22:6, added to the culture restores a fatty acid profile similar to controls (i.e. in vivo tissue taken at post-natal day 5). The concentrations were respectively 1 microgram 20:4/25 micrograms albumin/ml and 0,5 microgram/12,5 micrograms albumin/ml. Adding PUFA stimulates the proliferation of small dense cells by more than 200% of the control, as quantified by autoradiography of 3H-thymidine. These cells are not neurons, but possibly oligodendro...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.