Effect of Supporting Polyelectrolyte Multilayers and Deposition Conditions on the Formation of 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine/1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine Lipid Bilayers

Langmuir : the ACS Journal of Surfaces and Colloids
Magdalena WlodekMarta Kolasinska-Sojka

Abstract

The formation of complete supported lipid bilayers by vesicle adsorption and rupture was studied in relation to deposition conditions of vesicles and underlying cushion formed from various polyelectrolytes. Lipid vesicles were formed from zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and negatively charged 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) in phosphate buffer of various pH with or without NaCl addition. Polyelectrolyte multilayer films (PEM) were constructed by sequential adsorption of alternately charged polyelectrolytes from their solutions-layer-by-layer deposition (LBL). The mechanism of the formation of supported lipid bilayer on polyelectrolyte films was studied by quartz crystal microbalance with dissipation monitoring (QCM-D) and atomic force microscopy (AFM). QCM-D allowed following the adsorption kinetics while AFM measurements verified the morphology of lipid vesicles and isolated bilayer patches on the PEM cushions providing local topological images in terms of lateral organization. Additionally, polyelectrolyte cushions were characterized with ellipsometry to find thickness and swelling properties, and their roughness was determined using AFM. It has been demonstrat...Continue Reading

References

Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A A Brian, H M McConnell
Jan 7, 2003·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·E ReimhultB Kasemo
Aug 29, 2003·Biophysical Journal·Rainer A BöckmannHelmut Grubmüller
Oct 29, 2003·Biophysical Journal·Ralf RichterAlain Brisson
Apr 19, 2005·Bioelectrochemistry·Marta Kolasińska, Piotr Warszyński
Oct 31, 2006·Journal of Colloid and Interface Science·Marta KolasińskaPiotr Warszyński
May 17, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Brian R DorvelRandolph S Duran
Apr 23, 2008·The Journal of Physical Chemistry. B·Lars RennerCarsten Werner
May 27, 2008·Physical Chemistry Chemical Physics : PCCP·Soonwoo Chah, Richard N Zare
Aug 5, 2008·Journal of Colloid and Interface Science·M KolasińskaP Warszyński
May 14, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·M ElzbieciakP Warszyński
Aug 27, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Marta KolasinskaRumen Krastev
Sep 4, 2009·Colloids and Surfaces. B, Biointerfaces·Sandra GromelskiGerald Brezesinski
Dec 17, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Martin KaufmannTilo Pompe
Oct 22, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Kirk MulliganLinda J Johnston
Feb 8, 2013·International Journal of Molecular Sciences·Simon J AttwoodZoya Leonenko
Jun 8, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Andreia A DuarteMaria Raposo
Jun 27, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Gerald FindenigVolker Ribitsch

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