Effect of surface functionalizations of multi-walled carbon nanotubes on neoplastic transformation potential in primary human lung epithelial cells

Nanotoxicology
Todd A StueckleLiying Wang

Abstract

Functionalized multi-walled carbon nanotube (fMWCNT) development has been intensified to improve their surface activity for numerous applications, and potentially reduce toxic effects. Although MWCNT exposures are associated with lung tumorigenesis in vivo, adverse responses associated with exposure to different fMWCNTs in human lung epithelium are presently unknown. This study hypothesized that different plasma-coating functional groups determine MWCNT neoplastic transformation potential. Using our established model, human primary small airway epithelial cells (pSAECs) were continuously exposed for 8 and 12 weeks at 0.06 μg/cm2 to three-month aged as-prepared-(pMWCNT), carboxylated-(MW-COOH), and aminated-MWCNTs (MW-NHx). Ultrafine carbon black (UFCB) and crocidolite asbestos (ASB) served as particle controls. fMWCNTs were characterized during storage, and exposed cells were assessed for several established cancer cell hallmarks. Characterization analyses conducted at 0 and 2 months of aging detected a loss of surface functional groups over time due to atmospheric oxidation, with MW-NHx possessing less oxygen and greater lung surfactant binding affinity. Following 8 weeks of exposure, all fMWCNT-exposed cells exhibited signifi...Continue Reading

References

Oct 19, 2005·Toxicology Letters·Christie M SayesVicki L Colvin
Dec 21, 2010·International Journal of Hygiene and Environmental Health·Heidi BeckerMarike Kolossa-Gehring
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Oct 13, 2011·Environmental Science & Technology·Elijah J PetersenKai Loon Chen
May 16, 2012·Environmental Science & Technology·Gregory V LowryJamie R Lead
May 30, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Cinzia Lucia UrsiniSergio Iavicoli
Aug 14, 2012·Nanotoxicology·Dale W PorterVincent Castranova
Aug 1, 2013·Advanced Drug Delivery Reviews·Ken DonaldsonAnja Schinwald
Jan 11, 2014·Particle and Fibre Toxicology·Linda M SargentSteven H Reynolds
Feb 1, 2014·Particle and Fibre Toxicology·Katelyn J SiegristLinda M Sargent
Dec 17, 2014·The Lancet Oncology·Yann GrosseUNKNOWN International Agency for Research on Cancer Monograph Working Group
Dec 17, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Maria MrakovcicEleonore Fröhlich
Feb 24, 2015·Environment International·Denise M MitranoBernd Nowack
Mar 5, 2016·Chemical Reviews·Farid KhelifaPhilippe Dubois
Apr 1, 2016·Carbon·Shuji TsuruokaMorinobu Endo
Oct 16, 2016·Particle and Fibre Toxicology·Tatsuya KasaiShoji Fukushima

❮ Previous
Next ❯

Citations

May 8, 2018·Tissue Engineering. Part a·Rudra BhowmickHeather Gappa-Fahlenkamp
Mar 3, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sreepradha SridharanJames C Bonner
Apr 24, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·K KyriakidouC A Charitidis
Apr 24, 2020·Current Stem Cell Research & Therapy·Chen-Chen Mao, Xiaoxiao Cai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.