Effect of Surfactant Structure on the Superactivity of Candida rugosa Lipase

Langmuir : the ACS Journal of Surfaces and Colloids
Francesco GabrieleMatteo Tiecco

Abstract

In this work, we present the effects of ionic and zwitterionic surfactants on the hydrolytic activity of Candida rugosa lipase (CRL), one of the most important and widely used microbial lipases. A series of amine N-oxide surfactants was studied to explore the relationship between their molecular structures and their effect on catalytic properties of CRL. These zwitterionic amphiphiles are known for their ability to form aggregates that can increase their size, thanks to a sphere-rod transition, without any additive. Enzyme activity seemed to be improved by morphological changes of micelles from spherical to rod-like, and the structure of the monomers played a crucial role in this transition. In fact, all the amine oxides investigated provoked superactivation, but the CRL activity increased by lengthening the alkyl chain of N-oxide surfactants, whereas it decreased in the presence of bulky head groups. Superactivity was mainly because of an increase in kcat (0.57 s-1 in buffer, 0.80-1.99 s-1 in surfactant solutions) and, in some cases, a decrease in KM (2 × 10-3 M in buffer, 1.08-4.28 × 10-3 M in surfactant solutions). Micelles seemed to play a dual role: superactivity occurred at surfactant concentrations higher than their crit...Continue Reading

References

Apr 1, 1994·Protein Engineering·M LottiL Alberghina
Feb 13, 1993·Biochimica Et Biophysica Acta·L RúaA Ballesteros
Dec 19, 2000·Enzyme and Microbial Technology·B Orlich, R Schomäcker
Feb 24, 2001·Advances in Colloid and Interface Science·H Maeda, R Kakehashi
Oct 11, 2003·Biotechnology Advances·R SharmaU C Banerjee
Aug 12, 2004·Applied Biochemistry and Biotechnology·Alain HoudeDanielle Leblanc
Nov 3, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Hong-Tao DengPatrick Seta
Nov 18, 2005·Biotechnology Advances·Pablo Domínguez de MaríaAndrés R Alcántara
Aug 21, 2007·Advances in Colloid and Interface Science·María A BiasuttiEduardo A Lissi
Sep 14, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Laura GoracciGianfranco Savelli
Apr 21, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Piero BaglioniMatteo Tiecco
Sep 1, 2009·Bioresource Technology·Debajyoti GoswamiSirshendu De
Mar 17, 2010·Journal of Colloid and Interface Science·L BrinchiD Berti
Mar 15, 2011·Biochimica Et Biophysica Acta·Daniel Otzen
Mar 29, 2014·TheScientificWorldJournal·Shivika Sharma, Shamsher S Kanwar
May 18, 2016·Biotechnology Advances·Jorge BarriusoMaría Jesús Martínez
Jul 27, 2017·Chemical Reviews·Toshiyuki Itoh
Sep 21, 2017·Journal of Food Science and Technology·N Annapurna DeviR J Bhargavi
Dec 19, 2017·Colloids and Surfaces. B, Biointerfaces·Krister Holmberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.