Effect of T cell subset dose on outcome of T cell-depleted bone marrow transplantation

Bone Marrow Transplantation
Y KawanishiCarolyn Keever-Taylor

Abstract

T cell depletion using the murine monoclonal antibody (moAb) T10B9 is unique in that the T cell receptor (TCR)gamma delta bearing subset is relatively spared compared to the TCR alpha beta + subset. We evaluated the probabilities of engraftment, acute and chronic graft-versus-host disease (GVHD), relapse, and survival in 273 recipients of marrow T cell depleted using T10B9. Sixty-two patients received marrow from an HLA-identical sibling, 54 patients received partially matched related donor marrow and 157 patients received unrelated donor marrow. Limiting dilution analysis (LDA) was used to estimate total clonable T cell dose in all patients and a modified LDA using moAb-coated immunomagnetic beads was used to estimate TCR alpha beta +, CD4+, and CD8+ T cells in a subset of patients. TCR gamma delta + cell dose was estimated by flow cytometry. Cox proportional hazards regression models were used to assess the impact of T cell subset dose/kg of body weight on outcome. We found a significant association of TCR gamma delta + T cell dose (P = 0.004), but not TCR alpha beta + T cell dose or total clonable T cell dose, with the probability of engraftment. TCR alpha beta +, CD4+, CD8+ and total clonable T cell dose were significantly ...Continue Reading

Citations

Jul 27, 2001·British Journal of Haematology·M W LowdellH G Prentice
Jan 25, 2003·British Journal of Haematology·Sarah E LawsonPhilip J Darbyshire
Jul 21, 1998·Annals of the New York Academy of Sciences·A PigaB Dresow
Oct 28, 2008·Burns : Journal of the International Society for Burn Injuries·Martin G Schwacha
Nov 13, 2004·Stem Cells·Osnat Bohana-Kashtan, Curt I Civin
Jul 15, 2015·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Piyanuch KongtimStefan O Ciurea
Mar 8, 2013·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Ulas D BayraktarEsperanza B Papadopoulos
Mar 4, 2005·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Lawrence S Lamb, Richard D Lopez
Oct 14, 2009·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Nancy H CollinsDaniel J Weisdorf
Jul 5, 2017·Biomedicines·Haitham AbdelhakimAyman Saad
Feb 18, 2021·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Ran Reshef

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