Effect of tankyrase 1 on autophagy in the corpus cavernosum smooth muscle cells from ageing rats with erectile dysfunction and its potential mechanism.

Asian Journal of Andrology
Jian ZhangZhong-Cheng Xin

Abstract

This study compared tankyrase 1 expression and autophagy quantity between erectile dysfunction (ED) and non-ED rats' corpus cavernosum smooth muscle cells (CSMCs). This study aslo explored the effect and possible mechanism of tankyrase 1 on autophagy and cell proliferation in ageing ED rats' CSMCs. The intracavernous pressure and mean systemic arterial pressure were measured to investigate erectile function so that eight 24-month-old ED and eight 8-month-old male Wistar rats were chosen respectively. The rat CSMCs were isolated and cultured by enzyme digestion, in which tankyrase 1 expression and autophagy quantity were compared. Tankyrase 1 overexpression was induced with plasmid transfection by Lipofectamine. The effect of tankyrase 1 overexpression on proliferation, autophagy and mTOR pathway in 24-month-old ED rats' CSMCs was measured by the cell growth curve in MTT assay, cell cycle analysis in flow cytometry (FCM), key protein expression in Western blot, autophagy quantity in transmission electron microscopy, monodansylcadaverine staining and GFP-LC3 fluorescence. The primary CSMCs were confirmed by immunofluorescence, and the purity was 99.1% in FCM. Compared with that of 8-month-old rats, tankyrase 1 expression and auto...Continue Reading

References

Nov 1, 2000·Cell·T Schmelzle, M N Hall
May 22, 2003·The Journal of Biological Chemistry·Fatima LekmineLeonidas C Platanias
May 8, 2004·Experimental Gerontology·Alexei Terman, Ulf T Brunk
Aug 25, 2004·The International Journal of Biochemistry & Cell Biology·Alfred J Meijer, Patrice Codogno
Aug 25, 2004·The International Journal of Biochemistry & Cell Biology·Noboru Mizushima
Sep 15, 2005·Clinical Cornerstone·Aly Rashid
Sep 24, 2005·Cell·Sophie PattingreBeth Levine
Jan 5, 2006·International Journal of Impotence Research·M E Camacho, C A Reyes-Ortiz
Jan 25, 2006·European Urology·Francesco MontorsiArthur L Burnett
Feb 14, 2006·BJU International·Waldemar S CostaFrancisco J B Sampaio
Feb 25, 2006·The Journal of Investigative Dermatology·Anelisa B CampanerJeffrey R Morgan
Jul 29, 2006·Autophagy·Daniel J KlionskyPatrice Codogno
Apr 19, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Ryohei MiyazakiKenji Sunagawa
May 24, 2008·Journal of the American College of Cardiology·Ronald Ching-Wan MaPeter Chun-Yip Tong
Feb 19, 2009·Autophagy·Eisuke Itakura, Noboru Mizushima
Jun 13, 2009·Current Molecular Medicine·Kasper M A Rouschop, Bradly G Wouters
Jun 25, 2009·Autophagy·Young-Eun ParkIchizo Nishino
Feb 4, 2010·The EMBO Journal·Maria Chiara MaiuriGuido Kroemer
Feb 23, 2010·Infectious Disease Clinics of North America·Andrew J Stewardson, M Lindsay Grayson

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Citations

Aug 9, 2011·Asian Journal of Andrology·Wei-Ren LiZhong-Cheng Xin
May 1, 2012·The Journal of Biological Chemistry·Pooja AgrawalRobert E Hughes
Jun 8, 2017·The Aging Male : the Official Journal of the International Society for the Study of the Aging Male·Ecem KayaSerap Gur
Sep 3, 2020·European Journal of Medicinal Chemistry·Manoj G DamaleJaiprakash N Sangshetti
May 18, 2021·BioMed Research International·Ji-Sheng WangBin Wang

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