Effect of tear collection on lacrimal total protein content in dogs and cats: a comparison between Schirmer strips and ophthalmic sponges

BMC Veterinary Research
Lionel SebbagJonathan P Mochel

Abstract

Quantification of lacrimal total protein content (TPC) is an important tool for clinical scientists to understand disease pathogenesis, identify potential biomarkers and assess response to therapy, among other applications. However, TPC is not only affected by disease state but also by the method used for tear collection. Thus, the purpose of this study is to determine the impact on TPC of two methods of tear collection in dogs and cats: Schirmer strips and polyvinyl acetal (PVA) sponges. (i) In vivo - Ten healthy dogs and 10 healthy cats were examined. Each animal underwent two sessions, separated by 10 min, in which a Schirmer strip was placed in one randomly selected eye until the 20-mm mark was reached, while a strip of PVA sponge was placed in the other eye for 1 min. (ii) In vitro - Schirmer strips and PVA sponges were spiked with various volumes of four bovine serum albumin solutions (0.5, 4, 10, and 20 mg/mL). In both experiments, the wetted absorbent materials were centrifuged for 1 min, and the TPC was quantified on the extracted fluid using Direct Detect™ infrared spectroscopy. Lacrimal TPC in dogs and cats ranged from 5.2 to 14.6 mg/mL and from 6.2 to 20.6 mg/mL, respectively. In cats, TPC was significantly lower wi...Continue Reading

References

Jul 1, 1983·American Journal of Ophthalmology·A Berta
Jan 1, 1994·Advances in Experimental Medicine and Biology·A Kijlstra, A Kuizenga
Jan 1, 1994·Advances in Experimental Medicine and Biology·H J DavidsonP C Montgomery
Aug 24, 2000·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·V Ng, P Cho
Jan 12, 2007·The Ocular Surface·Alison M McDermott
Jan 1, 2008·Research in Veterinary Science·Carla de Freitas CamposMark D P Willcox
Aug 5, 2008·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Shimin LiNancy A McNamara
Feb 4, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Kaijun LiKaili Wu
Jan 29, 2011·Acta Ophthalmologica·Andrea PetznickDeborah F Sweeney
Feb 4, 2012·Journal of Proteomics·Éva CsőszJózsef Tőzsér
May 19, 2012·Current Eye Research·Tatsuya MimuraShiro Amano
May 29, 2012·Journal of Proteomics·Lei ZhouRoger W Beuerman
Jan 30, 2013·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Andreas PosaFriedrich Paulsen
Dec 25, 2015·PloS One·Mateusz WiniarczykStanislaw Winiarczyk
Aug 29, 2017·Veterinary Ophthalmology·Lionel SebbagJonathan P Mochel

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Citations

May 21, 2019·Veterinary Ophthalmology·András M KomáromyTerah R Webb
Aug 6, 2019·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Lionel SebbagJonathan P Mochel
Sep 14, 2020·Veterinary Ophthalmology·Hannah M TerhaarLionel Sebbag
May 28, 2020·Acta Veterinaria Scandinavica·Jéssica Fontes VelosoRenata Santiago Alberto Carlos
Apr 1, 2020·Lab on a Chip·Zachary BallardAydogan Ozcan
Oct 8, 2020·Translational Vision Science & Technology·Anis Barmada, Scott A Shippy
Oct 16, 2020·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Nayone Lima Lantyer-AraujoArianne Pontes Oriá
Jul 31, 2021·Veterinary Medicine International·Jéssica Fontes VelosoRenata Santiago Alberto Carlos
Dec 18, 2021·Frontiers in Medicine·Lionel SebbagJonathan P Mochel

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Methods Mentioned

BETA
tear
infrared spectrometry

Software Mentioned

SigmaPlot

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