Effect of temperature and loading on the structure of β-casein/ibuprofen assemblies

Journal of Colloid and Interface Science
Tanya TurovskyDganit Danino

Abstract

β-Casein is a 24 kDa amphiphilic and unstructured protein that self-assembles into small core-shell micelles at a wide range of concentrations, pH values and temperatures. We recently developed the micelles as nanocarriers for oral delivery of hydrophobic drugs. In this paper we examined the effect of the hydrophobic non-steroidal anti-inflammatory drug (NSAID) ibuprofen on the micellar structure, as a function of temperature and loading. Using cryo-transmission electron microscopy (cryo-TEM) we find two routes of organization – mixed micellization and co-assembly (aggregation). The time-dependent events that characterize the second routes has been examined in detail. At 25 °C we find coexistence of small assemblies and larger aggregates of irregular (but defined) structures that contain the drug. Increasing the drug loading increases the relative number of the larger aggregates and their dimensions, leading eventually to the formation of long then branched structures, like in amphiphilic block copolymer solutions. Similar trends were identified for changes in the temperature. Combined, our results suggest that ibuprofen acts as a co-surfactant that possibly is localizes to the interface rather than being encapsulated in the mi...Continue Reading

References

Oct 9, 2002·Trends in Biochemical Sciences·Peter Tompa
Feb 8, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Brian C StephensonDaniel Blankschtein
Nov 17, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Nily DanDganit Danino
Feb 29, 2008·Journal of Agricultural and Food Chemistry·Irina PortnayaDganit Danino
Apr 18, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Juan Li, Ping Yao
May 13, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Beth FosterYoussef Espidel
Jan 24, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Michal BacharDganit Danino
Jun 13, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Tanya TurovskyDganit Danino

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Citations

Jul 29, 2016·Physical Chemistry Chemical Physics : PCCP·Irina PortnayaDganit Danino
Jul 18, 2017·Topics in Current Chemistry·Tomasz Konrad Głąb, Janusz Boratyński
Feb 28, 2021·Colloids and Surfaces. B, Biointerfaces·Shixiong KangKe Wang
May 3, 2021·Advances in Colloid and Interface Science·Chuan-He Tang
Jan 18, 2020·Food Chemistry·Luis Gustavo Lima NascimentoAntonio Fernandes de Carvalho

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