Effect of temperature and pressure on yeast invertase stability: a kinetic and conformational study

Journal of Biotechnology
D Cavaille, D Combes

Abstract

Kinetics of the temperature- or pressure-induced denaturation of invertase from Saccharomyces cerevisiae were obtained in the temperature range 45-70 degrees C and in the pressure range 500-650 MPa. The investigation was done by measuring the residual activities after cooling or pressure release and the intrinsic fluorescence of aromatic amino-acids (tyrosine and tryptophan) upon excitation at 277 nm. The residual activity decreased exponentially as a function of time incubation according to a biphasic model either with pressure or temperature, whereas the fluorescence emission indicated a difference between these two parameters. When the enzyme was subjected to thermal treatment, the fluorescence of tyrosine and tryptophan decreased slowly, while after high-pressure treatment, these aromatic residues become more exposed to the aqueous solvent during unfolding, giving rise to a large decrease in fluorescence in the 330-340 nm region. Moreover, in the latter case, an enhancement of light scattering intensity showed changes in protein-protein interactions.

References

Sep 1, 1992·Biochemistry·S D SamarasingheJ Jonas
Aug 1, 1990·Journal of Protein Chemistry·R L RemmeleA Bieber
Jan 1, 1987·Annals of the New York Academy of Sciences·D CombesP Monsan
Jul 25, 1986·Biochimica Et Biophysica Acta·K FukushimaR Shimozawa
Jun 1, 1983·Archives of Biochemistry and Biophysics·F K ChuF Maley
Feb 1, 1983·Quarterly Reviews of Biophysics·G Weber, H G Drickamer
Apr 28, 1981·Biochemistry·A A Paladini, G Weber
Oct 1, 1981·Die Naturwissenschaften·K MüllerR Jaenicke
Oct 20, 1987·Biotechnology and Bioengineering·A Sadana, J P Henley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.