Effect of temperature on self-assembly of bovine beta-casein above and below isoelectric pH. Structural analysis by cryogenic-transmission electron microscopy and small-angle X-ray scattering

Langmuir : the ACS Journal of Surfaces and Colloids
Christian MoitziDganit Danino

Abstract

beta-Casein is one of the main proteins in milk, recently classified as an intrinsically unstructured protein. At neutral pH, it is composed of a highly polar N-terminus domain and a hydrophobic C-terminus tail. This amphiphilic block-copolymer-like structure leads to self-organization of the protein monomers into defined micelles. Recently, it has been shown that at room temperature, beta-casein also self-organizes into micelles in an acidic environment, but the effect of temperature on the micelles' formation and properties at the low pH regime were not explored. In the present study, we used two complementary techniques, cryogenic-transmission electron microscopy (cryo-TEM) and small-angle X-ray scattering (SAXS), to characterize at high-resolution the micelles' shape, dimensions, and aggregation numbers and to determine how these properties are affected by temperature between 1 and 40 degrees C. Two different regimes were studied: highly acidic pH where the protein is cationic, and neutral pH, where it is anionic. We found that flat disk-like micelles with low aggregation numbers formed at low temperature in the two pH regimes. Close to neutral pH increase in temperature involves a transition in the micelles' shape and dime...Continue Reading

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Citations

Jul 22, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Xiaoli L ZhangI Grillo
Sep 24, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Rekha Goswami ShresthaMasahiko Abe
Jan 26, 2011·Physical Chemistry Chemical Physics : PCCP·Fu-Gen WuZhi-Wu Yu
Jul 17, 2013·International Journal of Molecular Sciences·Shuren FengMarit Nilsen-Hamilton
Sep 18, 2012·Journal of Pharmaceutical and Biomedical Analysis·Sandra GrotefendHermann Wätzig
Jan 24, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Michal BacharDganit Danino
Feb 2, 2010·Colloids and Surfaces. B, Biointerfaces·Maria LundinMark W Rutland
Aug 25, 2009·Biochimica Et Biophysica Acta·Reza YousefiThomas Haertle
Nov 5, 2011·Chemistry, an Asian Journal·Teruyuki KomatsuChristoph Boettcher
Aug 20, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Ian M TuckerNick Hedges
Jun 13, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Tanya TurovskyDganit Danino
Mar 11, 2015·Journal of Colloid and Interface Science·Tanya TurovskyDganit Danino
Nov 17, 2010·Physical Chemistry Chemical Physics : PCCP·Irina PortnayaDganit Danino
Jul 29, 2016·Physical Chemistry Chemical Physics : PCCP·Irina PortnayaDganit Danino
May 20, 2017·Journal of Biomolecular Structure & Dynamics·Hamid DezhampanahLeila Hasani
Mar 3, 2021·Journal of Colloid and Interface Science·Prakram Singh ChauhanDganit Danino
Jan 7, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Frank ReinholdFranziska Gröhn
May 25, 2021·Food Science & Nutrition·Kong Yang WuQuan Yang Li
Jan 24, 2018·The Journal of Physical Chemistry. B·Ian W HamleyDganit Danino
Aug 18, 2021·Food Research International·Shuailing YangJacob J K Kirkensgaard

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