Effect of temperature on spore germination and vegetative cell growth of Clostridium botulinum.

Applied and Environmental Microbiology
N Grecz, L H Arvay

Abstract

Spore germination and vegetative growth of Clostridium botulinum type E strain VH at 2 to 50 degrees C were studied. At all of these temperatures, germination began immediately after the addition of the spores to the germination medium. Microscopic observations during germination revealed three types of spores: phase bright (ungerminated), phase variable (partially germinated), and phase dark (fully germinated). At all temperatures except 50 degrees C, there was a pronounced lag between the initial appearance of phase-variable spores and their eventual conversion to phase-dark spores. The number of partially germinated spores increased steadily, reaching 40 to 60% by 18 to 21 h of incubation. During this time, phase-dark, fully germinated spores developed slowly and did not exceed 28% in any of the samples. At 18 to 26 h of incubation, the rate of full germination increased abruptly four-fold. There was extensive and relatively rapid germination at 2 degrees C, the lowest temperature tested, yielding about 60% phase-variable spores by 18 h, which became phase-dark by 26 h of incubation. The optimum temperature for partial and full germination was consistently 9 degrees C. Germination at 50 degrees C was exceptionally rapid and ...Continue Reading

References

Jun 1, 1975·Bacteriological Reviews·R Y Morita
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Apr 1, 1980·Applied and Environmental Microbiology·H H Huss

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Citations

Jun 16, 2011·Luminescence : the Journal of Biological and Chemical Luminescence·Shou-Lei YanQing-Zhang Wang
Jan 6, 2009·Journal of the American Veterinary Medical Association·Anna Catharina B BergeDonald J Klingborg
Jun 10, 2010·Foodborne Pathogens and Disease·Reha Onur Azizoglu, Sophia Kathariou
Jan 1, 1990·Critical Reviews in Food Science and Nutrition·K StammenF Caporaso
Oct 6, 2017·Journal of Applied Microbiology·L J KohlerC K Cote

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