Effect of temperature on the retention of ionizable compounds in reversed-phase liquid chromatography: application to method development

Journal of Chromatography. a
Sabine HeinischJean-Louis Rocca

Abstract

The analysis of pharmaceutical compounds is often a difficult challenge which requires mathematical tools to improve the quality of the separation method. This work is an attempt to rationalize the anomalous variation of the logarithm of the retention factor with temperature in case of ionizable compounds. The effect of temperature on ionizable compounds was studied within a large range of temperature, ranging from 30 to 130 degrees C. The determination of the so-called chromatographic pKa and the study of its variation with temperature allow to explain why the forms of the van't Hoff curves are so different depending on the type of solute, the type of buffer and the type of the mobile phase. A retention model along with a computation procedure is proposed to optimize both temperature and mobile phase composition and to provide good and robust conditions as shown by illustrative examples.

Citations

Dec 10, 2009·Analytical and Bioanalytical Chemistry·Davy GuillarmeJean-Luc Veuthey
Apr 6, 2012·Journal of Pharmaceutical and Biomedical Analysis·Szabolcs FeketeDavy Guillarme
Sep 15, 2010·Journal of Pharmaceutical and Biomedical Analysis·Jennifer M CunliffeJim X Shen
Oct 9, 2009·Journal of Chromatography. a·C WestM Lafosse
Dec 19, 2008·Journal of Chromatography. a·Sabine Heinisch, Jean-Louis Rocca
Nov 18, 2008·Journal of Chromatography. a·Colin F Poole, Salwa K Poole
Oct 8, 2008·Journal of Chromatography. a·P Nikitas, A Pappa-Louisi
May 27, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wenyun LuJoshua D Rabinowitz
Sep 4, 2007·Journal of Chromatography. a·Dao T-T NguyenJean-Luc Veuthey
Nov 30, 2006·Journal of Chromatography. a·Davy GuillarmeJean-Luc Veuthey
Feb 29, 2008·Journal of Separation Science·Dao T-T NguyenJean-Luc Veuthey
Jul 12, 2007·Journal of Separation Science·Uwe D Neue
Sep 22, 2011·Journal of Separation Science·Endler M Borges, Carol H Collins
Sep 8, 2015·Analytica Chimica Acta·Leonardo G GagliardiCecilia B Castells
Aug 21, 2013·Journal of Chromatography. a·Manish M KhandagalePaul R Haddad
May 22, 2007·Analytical Chemistry·R K Gilpin, C S Gilpin

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